I think its hargobind khurana.
Cutting the gene out of DNA with enzymes - gradpoint
Insert the gene into bacteria. -Apex
It was first isolated in the liver in 1933.
A gene isolated from a bacterium that produces a crystalline protein that when eaten by insects shreds their insides kills them. Acts as an effective biological alternative to pesticide for this reason.
The production of recombinant DNA involves several key steps: First, a specific gene of interest is isolated using restriction enzymes that cut DNA at particular sequences. Next, this gene is inserted into a plasmid vector, which also contains a marker gene for selection. The recombinant plasmid is then introduced into a host organism, often bacteria, through a process called transformation. Finally, the host cells replicate the plasmid, producing multiple copies of the recombinant DNA and expressing the inserted gene.
You isolate the gene in genetic engineering by first locating the gene you wish to be isolated. Then you use a restrictive enzyme to isolate it, and lastly take the gene out
isolated gene pools
Mutations
A single abnormal gene produces HD. In 1993, scientists finally isolated the HD (Huntington's Chorea) gene on chromosome 4.
bacteria
Cutting the gene out of DNA with enzymes - gradpoint
Robert Koch was the person who first isolated cholera in 1883
It was first isolated in 1895 by William Ramsay.
Insert the gene into bacteria. -Apex
The cDNA (complementary DNA) encoding the eukaryotic protein must first be isolated from the cell prior to cloning. This involves reverse transcription of the messenger RNA (mRNA) and subsequent amplification to obtain the gene of interest for cloning into a bacterial expression vector.
The first virus to be isolated and identified was the tobacco mosaic virus in 1892 by Russian scientist Dmitri Ivanovsky.
It was first isolated in the liver in 1933.