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Absorbance at 750 nm in Lowry's method is used because it corresponds to the peak absorbance of the copper-tyrosine complex formed during the reaction, ensuring accurate measurement of the protein concentration. This wavelength specifically targets the color change associated with the biuret reaction, enhancing the sensitivity and specificity of the assay.

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What are two reasons why absorbance measurements are taken at lambda max?

Absorbance measurements are taken at λ max because it represents the wavelength at which a compound absorbs light most strongly, allowing for more accurate and sensitive detection of the compound. Additionally, λ max provides specific information about the electronic structure of the compound, aiding in its identification and characterization.


What measures should be taken to avoid the threat of rodents?

what measures should be taken to avoid the threats of rodents


What are the precautions to be taken in the beer Lambert experiment?

In the Beer-Lambert experiment, it is essential to ensure that the cuvettes used are clean and free of scratches to avoid scattering of light. The light source should be stable and properly aligned with the detector to ensure accurate measurements. Additionally, the solution concentration must be within the linear range of the Beer-Lambert law, and the path length of the cuvette must be consistent throughout the experiment. Lastly, all measurements should be conducted at a constant temperature to prevent variations in absorbance.


How can the speed of sound be determined by the method of echo?

The speed of sound can be determined using the method of echoes by measuring the time it takes for a sound wave to travel to a reflective surface and back. By dividing the total distance traveled by the time taken, one can calculate the speed of sound as twice the distance divided by the time taken. This method is commonly used in environments where direct sound measurements are difficult, such as in large open spaces or underwater.


What happen when petrol is in taken?

stupids i asked the question, then why should i answer it

Related Questions

What are two reasons why absorbance measurements are taken at lambda max?

Absorbance measurements are taken at λ max because it represents the wavelength at which a compound absorbs light most strongly, allowing for more accurate and sensitive detection of the compound. Additionally, λ max provides specific information about the electronic structure of the compound, aiding in its identification and characterization.


Why are colored belts worn in karate?

They indicate progression in the arts. They were taken from Judo and their traditions. For the instructor they help them know what skills the student should know and what they should be learning. For the students they provide a method of reward.


An approach taken to solve a problem in science?

scientic method


Volume of blood taken in wintrobe method?

2 ml


What method for passing a bill has never been taken?

ratification


What an approach taken to solve a problem in science?

The Scientific Method :)


What is the empirical method?

Empirical method is generally taken to mean the collection of data on which to base a theory or derive a conclusion in science.


A method of making a map in which aerial photographs of the area are taken?

photogrammetry


When should gabapenten be taken?

Gabapentin should be taken exactly as prescribed by a doctor.


The part of an experiment in which you outline the steps taken to perform an experiment?

The scientific method


From what religion was an effective modern method of protest taken?

Judaism


Which trail shoes are available at sale prices?

I have taken this method first because i think this is the most popular method/way of hacking facebook. I studied various facebook surveys taken on web about hacking facebook. The results of these surveys show Phishing as the most used method to hack facebook and to note_Phishing is favorite method of facebook hackers.