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Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
Why is anthracene is used for calibration of hplc?
Anthracene is used as a calibration standard in High Performance Liquid Chromatography (HPLC) because it has a well-defined retention time and peaks in the UV-visible spectrum, making it easy to detect and quantify. Its consistent behavior helps in determining retention times, resolving power, and column efficiency during method development and troubleshooting in HPLC.
How separated nadh nad nadph and nadp in hplc?
In HPLC, NADH, NADPH, and NADP can be separated using a reverse-phase column with a suitable mobile phase, often involving a combination of water and organic solvents like acetonitrile or methanol. The differences in their charge and polarity allow for distinct retention times; NADH and NADPH are typically retained longer due to their reduced forms and higher polarity. A gradient elution can enhance separation, while UV detection at specific wavelengths helps in identifying and quantifying each nucleotide. Proper sample preparation and pH adjustment of the mobile phase can further improve resolution.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
Why acetone used in hplc calibration?
UV cut off of acetone is 330 nm and it is easyly available,non expensive. you can use any other solvent which has UV cut off above 250.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
What is difference between an HPLC UV detector and a fluorescence detector?
HPLC UV detectors measure absorbance of UV light at a specific wavelength, while fluorescence detectors measure the emission of light at a longer wavelength after excitation with UV light. Fluorescence detectors are more sensitive and selective than UV detectors, but may require additional steps such as derivatization for certain compounds.
How can you detect U.V. from sun?
UV from sun could be detected using UV sensors. The technology has been that advanced.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
Why is anthracene is used for calibration of hplc?
Anthracene is used as a calibration standard in High Performance Liquid Chromatography (HPLC) because it has a well-defined retention time and peaks in the UV-visible spectrum, making it easy to detect and quantify. Its consistent behavior helps in determining retention times, resolving power, and column efficiency during method development and troubleshooting in HPLC.
How separated nadh nad nadph and nadp in hplc?
In HPLC, NADH, NADPH, and NADP can be separated using a reverse-phase column with a suitable mobile phase, often involving a combination of water and organic solvents like acetonitrile or methanol. The differences in their charge and polarity allow for distinct retention times; NADH and NADPH are typically retained longer due to their reduced forms and higher polarity. A gradient elution can enhance separation, while UV detection at specific wavelengths helps in identifying and quantifying each nucleotide. Proper sample preparation and pH adjustment of the mobile phase can further improve resolution.
How are ultraviolet waves detected?
Ultraviolet waves are detected using instruments like photodetectors or spectrometers that are sensitive to UV radiation. These devices can measure the intensity of UV light and provide information about its wavelength and frequency. UV detection is important for various applications including scientific research, environmental monitoring, and medical diagnostics.
Why cholroform is rarely used as solvent in recording uv spectrum?
Chloroform is rarely used as a solvent for recording UV spectra because it has significant absorbance in the UV range, particularly around 240 nm, which can interfere with the analysis of samples. This absorbance can obscure the spectral features of the analyte, leading to inaccurate results. Additionally, chloroform can be toxic and poses safety risks, prompting the use of more suitable solvents like methanol or acetonitrile that have lower UV absorbance in the relevant range.
