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Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
Why is anthracene is used for calibration of hplc?
Anthracene is used as a calibration standard in High Performance Liquid Chromatography (HPLC) because it has a well-defined retention time and peaks in the UV-visible spectrum, making it easy to detect and quantify. Its consistent behavior helps in determining retention times, resolving power, and column efficiency during method development and troubleshooting in HPLC.
Could the presence of different pigments be detected before separation by chromatography?
Yes, the presence of different pigments can be detected before separation by chromatography through techniques such as UV-Vis spectroscopy or fluorescence spectroscopy. These techniques can provide information about the absorption or emission properties of the pigments present in a sample.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
Why acetone used in hplc calibration?
UV cut off of acetone is 330 nm and it is easyly available,non expensive. you can use any other solvent which has UV cut off above 250.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
What is difference between an HPLC UV detector and a fluorescence detector?
HPLC UV detectors measure absorbance of UV light at a specific wavelength, while fluorescence detectors measure the emission of light at a longer wavelength after excitation with UV light. Fluorescence detectors are more sensitive and selective than UV detectors, but may require additional steps such as derivatization for certain compounds.
How can you detect U.V. from sun?
UV from sun could be detected using UV sensors. The technology has been that advanced.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
Why is anthracene is used for calibration of hplc?
Anthracene is used as a calibration standard in High Performance Liquid Chromatography (HPLC) because it has a well-defined retention time and peaks in the UV-visible spectrum, making it easy to detect and quantify. Its consistent behavior helps in determining retention times, resolving power, and column efficiency during method development and troubleshooting in HPLC.
How are ultraviolet waves detected?
Ultraviolet waves are detected using instruments like photodetectors or spectrometers that are sensitive to UV radiation. These devices can measure the intensity of UV light and provide information about its wavelength and frequency. UV detection is important for various applications including scientific research, environmental monitoring, and medical diagnostics.
How does an HPLC work to separate and analyze compounds in a sample?
High Performance Liquid Chromatography (HPLC) works by using a liquid solvent to separate compounds in a sample based on their interactions with a stationary phase. The sample is injected into a column where the compounds travel at different speeds, allowing for separation. Detection methods like UV spectroscopy or mass spectrometry are then used to analyze and quantify the separated compounds.
What difference between pda and uv detector in hplc?
PDA - Photo diode array UV- Ultra violet with use of PDA detector, we can measure the area or height of particular peak at different wavelengths ranging from 200 to 800 nm by injecting the solution at once. Where as in uv detector we can measure the area or height of the peak only at two different wavelengths. But that wavelengths also to be selected before injecting the solution.
