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What else can I help you with?
How can you verify the purity of bacteria?
The only way i can think of is using a spread plate technique to observe what grows on an agar plate. Capillary electrophoresis I believe is use to split biological samples (and chemical) based on charge and size.
Describe the aseptic technique that would be used when flooding the agar plate with bacteria?
An agar plate was flooded with a culture of a species of bacterium usually found in the mouth. Four steriled paper discs, A, B, C, and D, each containing a different brand of mouthwash, were then placed on the agar plate. The drawing shows the appearance of the plate after it had been incubated below body temperature for three days, this is to ensure that the bacteria are not harmful to humans. Describe the aseptic technique that would be used when flooding the agar plate with bacteria
Why must we use sterile techniques to incubate agar plates?
Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
Why do you use saline water when preparing a plate with blood agar?
Saline water is used to dilute the blood agar medium to control the osmotic environment, ensuring the optimal growth conditions for certain bacteria that may be sensitive to higher salt concentrations. This helps facilitate the growth of a wider range of bacteria on the plate.
How can you verify the purity of bacteria?
The only way i can think of is using a spread plate technique to observe what grows on an agar plate. Capillary electrophoresis I believe is use to split biological samples (and chemical) based on charge and size.
What is the significance of using a 'dry and hard' agar plate in streak plating?
Why is it impotant to use dry and hard agar for streaking
Describe the aseptic technique that would be used when flooding the agar plate with bacteria?
An agar plate was flooded with a culture of a species of bacterium usually found in the mouth. Four steriled paper discs, A, B, C, and D, each containing a different brand of mouthwash, were then placed on the agar plate. The drawing shows the appearance of the plate after it had been incubated below body temperature for three days, this is to ensure that the bacteria are not harmful to humans. Describe the aseptic technique that would be used when flooding the agar plate with bacteria
How do you recognize bacteria on agar plate and not confusing bacteria with eukaryotes like yeast or fungi?
Use selective media agar plates. Different types of agar will let bacteria grow and inhibit fungal growth, or vice versa.
Why must we use sterile techniques to incubate agar plates?
Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.
What are the differences between streak plate technique and pour plate technique?
Streak Plate:Pure colonies of bacterial or other microorganisms can be obtained on petri dishes by streak plate. The microbial mixture is transfered to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in one of several patterns. After the first sector is streaked in dish, the inoculating loop is sterlized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cells will drop from it as it is rubbed along the agar surface. These develop into seprate colonies.Pour Plate:Extensively used with procaryotes and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. Then small volumes of several diluted samples are mixed with liquid agar that has been cooled to about 45oC and the mixture are poured immediately into sterile culture dishes. Most bacteria and fungi are not killed by a brief exposure to the warm agar. After the agar has hardened each cell is fixed in place and forms an individual colony.
Prewarm agar plates?
To prewarm agar plates, simply place them in a 37°C incubator for about 30 minutes before use. This ensures that the agar solidifies evenly and prevents condensation from forming on the plates when they are inoculated. Always handle prewarmed plates carefully to maintain sterility.
What specific medium must be use in testing the effectiveness of antibiotics?
A growth medium must be used. The most common is Mueller-Hinton agar, but potato dextrose agar or other growth media could also be used.
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
What happens to a plate if agar is too hot?
You may kill your bacteria. To avoid this problem we "defrost" the agar in the microwave for 3-8 minutes (depending on how much you have) to liquefy it. It is important to loosen the cap of the agar container so that it doesn't explode in the microwave--never heat a closed system. When the agar comes out of the microwave it is too hot to plate with without the risk of killing your bacteria. Kepp the agar liquid by leaving in a 60 degree C water bath. When the temperature of the agar equilibrates with the bath it should be safe to use.
How can one accurately count colonies on an agar plate?
To accurately count colonies on an agar plate, one should use a colony counter or a grid system to track and tally individual colonies. It is important to ensure the plate is properly labeled and incubated under the correct conditions to allow colonies to grow distinctively. Counting should be done systematically and consistently to avoid errors.
Why do you use saline water when preparing a plate with blood agar?
Saline water is used to dilute the blood agar medium to control the osmotic environment, ensuring the optimal growth conditions for certain bacteria that may be sensitive to higher salt concentrations. This helps facilitate the growth of a wider range of bacteria on the plate.
