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RF values, or retention factors, can differ due to several factors, including the composition of the stationary and mobile phases in chromatography, the temperature during the experiment, and the nature of the analytes being separated. Variations in solvent polarity, pH, and concentration can also influence how substances interact with the stationary phase, leading to different RF values. Additionally, experimental conditions such as the type of chromatography method used (e.g., TLC, HPLC) can further contribute to these differences.

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Can 2 substances with different Rf values still be similar?

Yes, two substances with different Rf values can still be similar. Differences in Rf values can be due to various factors, such as the polarity of the compound or the composition of the stationary phase. Therefore, substances with different Rf values can still share similar chemical structures or functional groups.


Arrange the following compounds in order of increasing Rf values in TLC Anthracene benzil tryphenylmethanol?

The order of increasing RF values in TLC is Benzil, methanol anthracene and tryphenyl.


What are the Rf values of amino acids?

The Rf (retention factor) values of amino acids can vary based on the specific conditions used in thin layer chromatography. Generally, Rf values for different amino acids will fall within a range of approximately 0.1 to 0.9, with each amino acid having a unique Rf value based on its individual chemical properties. For precise values, it is best to consult specific experimental data or reference sources.


If a chromatogram is run for a longer period of timem will the Rf values increase for each pigment?

No, the Rf values will not increase for each pigment with a longer chromatography run time. The Rf value is a constant characteristic of a compound in a particular solvent system and is not affected by the duration of the chromatography run.


Would RF values of amino acid you use in paper chromatography be the same if you use another solvent system?

No, RF values depend on the specific solvent system used in the chromatography process. Changing the solvent system can alter the interactions between the amino acids and the stationary phase, resulting in different RF values. It is important to optimize the solvent system to achieve accurate and reproducible results.

Related Questions

Why are Rf values used?

Rf values are used mainly for simplicity. These values are generally easier to use to obtain percentages between a couple objects instead of using values that are already established.


Can 2 substances with different Rf values still be similar?

Yes, two substances with different Rf values can still be similar. Differences in Rf values can be due to various factors, such as the polarity of the compound or the composition of the stationary phase. Therefore, substances with different Rf values can still share similar chemical structures or functional groups.


How are Rf values used in real life?

Rf values can be used to identify the type of drugs that had been taken by those who went through drug overdose.


What are the significance of values?

Rf Values determine the solubility of a substance with respect to a certain solvent.


Arrange the following compounds in order of increasing Rf values in TLC Anthracene benzil tryphenylmethanol?

The order of increasing RF values in TLC is Benzil, methanol anthracene and tryphenyl.


What are the Rf values of amino acids?

The Rf (retention factor) values of amino acids can vary based on the specific conditions used in thin layer chromatography. Generally, Rf values for different amino acids will fall within a range of approximately 0.1 to 0.9, with each amino acid having a unique Rf value based on its individual chemical properties. For precise values, it is best to consult specific experimental data or reference sources.


Why Ni positive 2 and iron positive 3 ions have different Rf values?

Ni2+ and Fe3+ ions have different Rf values because they have different charge-to-size ratios, leading to differences in their interactions with the stationary phase in the chromatography process. The higher charge of Fe3+ compared to Ni2+ results in stronger electrostatic interactions with the stationary phase, causing it to move slower and have a higher Rf value.


How would you expect Rf values to change if you went from hexane-ethyl acetate solvent system to ethyl acetate only?

If you switch from a hexane-ethyl acetate solvent system to ethyl acetate only, you would expect the Rf values to decrease. Ethyl acetate is a more polar solvent than hexane, so compounds will interact more with the solvent and have shorter distances of travel on the TLC plate, resulting in lower Rf values.


If a chromatogram is run for a longer period of timem will the Rf values increase for each pigment?

No, the Rf values will not increase for each pigment with a longer chromatography run time. The Rf value is a constant characteristic of a compound in a particular solvent system and is not affected by the duration of the chromatography run.


How are Rf values used?

Rf value to compare the "unknown" components of colored candy dyes with the "known" components of food coloring dyes.


If two samples have identical Rf values does this mean that they are necessarily identical molecules Explain?

No. The molecules MAY (and probably are if you are presented with this situation in school/college lab courses) be identical but just the Rf values is not enough information to determine, you must conduct additional tests. Two different molecules can have the same Rf value.Compound A will always have an Rf of X in solvent M. Compound B will always have and Rf of Y in solvent M. But, Rf X can be equivalent to Rf Y without compounds A and B being identical.


What is the risk-free rate if the expected return is 20.4 and beta is 1.6 and expected market return is 15?

expected market return = risk free + beta*(market return - risk free) So by putting in values: 20.4 = rf+ 1.6(15-rf) expected market return = risk free + beta*(market return - risk free) So by putting in values: 20.4 = rf+ 1.6(15-rf) where rf = risk free 20.4 - 24 = rf - 1.6rf -3.6 = -0.6rf rf = 6