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Hi

what you incubate is generally E.coli at 37C. The optimum growth temperature for this bacteria is 37, although if you are expressing some specific recombinant protein the induction temperature may very depending upon the promoter of recombinant gene, but the general temp for bacterial growth is 37C.

There are other bacteria like Listeria Monocytogenes which grow at 8C, also, after appropriate growth of E.coli sometimes you change temperature or can do other things depending upon you promoter for recombinant gene in it.

So bottom line generally 37C for growth, but also depends on bacteria in very few cases for purpose of biotechnology.

hope this will help you.

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