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Check the ingredient i.e. salt concentration and also check whether the blood cell get lysed as some of the microbes need lysed blood cell and some need intact so depends upon needs of bacteria check your case and also see if you have taken a culture which should not be too old.
What else can I help you with?
What type of media is used for growing a culture from a throat specimen?
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
Why do you suppose that plate counts for urine infections are made from the blood agar plate not the MAC plate?
Plate counts for urine infections are typically made from blood agar plates rather than MacConkey (MAC) plates because blood agar supports the growth of a wider range of bacteria, including fastidious organisms that may not grow well on MAC plates. Blood agar provides the necessary nutrients and allows for the detection of hemolytic activity, which can be important for identifying certain pathogens. Additionally, urine samples often contain mixed flora, and blood agar can help in isolating and quantifying the relevant pathogens more effectively.
What is responsible for solidifying nutrient agar plates?
Agar, a type of polysaccharide derived from seaweed, solidifies nutrient agar plates when it cools below 45°C. This process forms a gel matrix that helps to support bacterial growth on the surface of the plate.
What is Proteus vulgaris on the Blood Hemolysis Test?
Proteus vulgaris is a bacterium that shows beta hemolysis on blood agar plates. In the Blood Hemolysis Test, this means that Proteus vulgaris will cause complete lysis of red blood cells, resulting in a clear zone around the bacterial growth on the agar plate.
What type of hemolysis e coli show on blood agar is it alpha beta or gama hemolysis?
E. coli typically does not demonstrate hemolysis on blood agar plates. It usually appears as non-hemolytic or gamma hemolysis, where there is no change in the red blood cells surrounding the bacterial growth.
What type of media is used for growing a culture from a throat specimen?
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
Types of agar plates and their function?
Nutrient agar plates: used for general growth of most bacteria. Blood agar plates: used to differentiate bacteria based on their ability to hemolyze red blood cells. MacConkey agar plates: used to differentiate lactose-fermenting bacteria from non-lactose fermenters based on their ability to grow and ferment lactose.
How do you store sheep's blood agar plates?
in the fridge
A common medium used for growing fastidious bacteria is?
blood agar, as it provides essential nutrients and growth factors needed for the growth of fastidious bacteria. Additionally, the presence of blood in the agar helps to support the growth of these bacteria by providing additional nutrients.
Why do you suppose that plate counts for urine infections are made from the blood agar plate not the MAC plate?
Plate counts for urine infections are typically made from blood agar plates rather than MacConkey (MAC) plates because blood agar supports the growth of a wider range of bacteria, including fastidious organisms that may not grow well on MAC plates. Blood agar provides the necessary nutrients and allows for the detection of hemolytic activity, which can be important for identifying certain pathogens. Additionally, urine samples often contain mixed flora, and blood agar can help in isolating and quantifying the relevant pathogens more effectively.
Why are agar plates stored inverted need two answers?
Storing agar plates inverted helps prevent condensation from collecting on the agar surface, which could interfere with bacterial growth or lead to contamination. Storing agar plates inverted also prevents any moisture present in the lid from dripping onto the agar surface, which can disrupt the growth of the bacterial colonies.
How can one identify contamination in agar plates?
Contamination in agar plates can be identified by observing the presence of unexpected growth, such as different colored colonies, fuzzy or irregular growth patterns, or growth outside of the designated area. Additionally, any unusual odors or textures on the agar surface may also indicate contamination.
What is responsible for solidifying nutrient agar plates?
Agar, a type of polysaccharide derived from seaweed, solidifies nutrient agar plates when it cools below 45°C. This process forms a gel matrix that helps to support bacterial growth on the surface of the plate.
What is Proteus vulgaris on the Blood Hemolysis Test?
Proteus vulgaris is a bacterium that shows beta hemolysis on blood agar plates. In the Blood Hemolysis Test, this means that Proteus vulgaris will cause complete lysis of red blood cells, resulting in a clear zone around the bacterial growth on the agar plate.
What type of hemolysis e coli show on blood agar is it alpha beta or gama hemolysis?
E. coli typically does not demonstrate hemolysis on blood agar plates. It usually appears as non-hemolytic or gamma hemolysis, where there is no change in the red blood cells surrounding the bacterial growth.
How will you check the sterility of the media?
Agar is a medium, so you are checking the sterility of the agar. After preparation one usually places an agar plate at room temperature and another agar plate at 35 to 37 degrees C. After 24-48 hrs of incubation a visual check is made to see if there is any visible growth on the uninoculated plates. If you are adding blood, etc. to the agar, those components can be checked by subbing them to a blood agar plate to see if there is any growth--which would indicate non-sterile components.
What are the problems with agar plates?
Agar plates can become contaminated with unwanted microorganisms, leading to inaccurate results. They can also dry out, affecting the growth of bacteria. Additionally, agar plates can become overcrowded with bacterial colonies, making it difficult to differentiate and count individual colonies.
