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Calcium chloride treatment in bacterial transformation is necesaary because the bacterial cells are permeable to chloride ions but not the calcium ions. This chloride intake will accompany along with influx of water into the cells which causes the cells to swell. This swelling followed by a mild heat treatment for about 2 minutes will induce the heat shock proteins which allows the uptake of the recombinant DNA!!!! Calcium is a divalent cation. It binds to the hydrophobic membrane and creates channels for uptake of foreign DNA.It can bind to both negatively charged hydrophilic DNA and the cell membrane, which is also has a negative charge. This enhances ability of the competence cell to take up the DNA.

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Why are bacteria treated with calcium ions and subjected to temperature shock?

Bacteria are treated with calcium ions and subjected to temperature shock to make them more permeable to foreign DNA, allowing for easier transformation with new genetic material. The calcium ions help destabilize the bacterial cell wall, while the temperature shock creates temporary pores in the membrane, facilitating the uptake of DNA molecules.


What ions are in calcium chloride?

Calcium chloride consists of calcium ions (Ca2+) and chloride ions (Cl-).


How many calcium ions in one mole?

A mole of calcium has 6.022 X 1023 calcium ions.


A mineral that contains calcium carbonate will provide a positive response to a test for .?

acid reaction


Formula for the compound formed by calcium ions and chloride?

Calcium chloride is the compound formed by calcium ions and chloride ions. Its chemical formula is CaCl2.


Is Calcium sulfate a base?

No, calcium sulfate is not a base. It is a salt composed of calcium ions and sulfate ions.


Do calcium ions react with sulphuric acid?

Yes, calcium ions (Ca2+) can react with sulfuric acid (H2SO4) to form calcium sulfate (CaSO4) and water (H2O). This is a double displacement reaction where the calcium ions in the calcium sulfate are derived from the calcium hydroxide that is formed after the initial reaction between calcium ions and sulfuric acid.


What ions are present in what ratio in a solution of aqueous calcium chloride?

In an aqueous solution of calcium chloride, the ions present are Ca²⁺ (calcium) and Cl⁻ (chloride) in a 1:2 ratio. This means for every calcium ion, there are two chloride ions present in the solution.


What are the ions for calcium fluoride?

The individual ions for calcium fluoride have the formulas Ca+2 and F-1 respectively. That means that in any sample of calcium fluoride, there must be twice as many of the fluoride ions.


What is the source of mineral elements like calcium that bacteria obtain in nutrient broth?

Calcium ions are often added to nutrient broths as a supplement for bacterial growth. This can be in the form of calcium chloride or other calcium salts. Bacteria require calcium for various cellular processes, so it is important to include it in the growth medium to support their growth and metabolism.


Is calcium chloride a sulfite?

No, calcium chloride is CaCl2 containing calcium ions (Ca2+) and chloride ions (Cl-). A sulfite must contain sulfite ions (SO32-)


What is calcium ions and chloride ions formula?

The ions should form CaCl2 because the two chlorine atoms each take an electron from calcium to form the chloride ion Cl-, and the calcium becomes Ca2+ ion.