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In a light microscope, you cannot focus both threads at once because of the limitation of depth of field. The microscope's optics create a specific plane of focus, and if two threads are at different depths, only one can be in sharp focus at a time. Adjusting the focus for one thread will inevitably cause the other to appear blurred. This limitation is inherent in optical systems and is influenced by factors such as aperture size and wavelength of light used.

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1w ago

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