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Why is the streak plate preferred over the spot inoculations in this procedure?
The streak plate method is preferred over spot inoculations because it allows for the isolation of individual colonies from a mixed culture, promoting the separation of different microorganisms. This technique creates a gradient of dilution across the agar plate, enabling the growth of distinct colonies that can be easily identified and characterized. Additionally, the streak plate method minimizes the risk of contamination and provides a more systematic approach to isolating pure cultures.
Which advance resulted from the development of a culture technique for muscle cells?
insulin production
Which is more rapid in proving the purity of a culture on a agar and WHY?
The streak plate method is more rapid in proving the purity of a culture on agar. This technique allows for the isolation of individual colonies from a mixed culture by diluting the sample across the surface of the agar plate. As the cells spread, they grow into separate colonies, enabling quick visual assessment of purity, usually within 24 to 48 hours. In contrast, methods like serial dilution can be more time-consuming and require additional steps to confirm purity.
How do calculate Minimum inhibition concentration for Antibiotics?
Minimum Inhibitory Concentration (MIC) for antibiotics is determined using methods like broth dilution or agar dilution. In the broth dilution method, a series of test tubes containing a culture medium and varying concentrations of the antibiotic are inoculated with the microorganism. The MIC is the lowest concentration of the antibiotic where no visible growth occurs after incubation. Alternatively, the agar dilution method involves incorporating different antibiotic concentrations into agar plates and observing the growth inhibition zone.
What is a ten fold serial dilution?
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
What is the actual absorbancy if 2mL of a culture is diluted by adding 4mL of water and the absorbancy reading of the diluted culture is 0.059?
The actual absorbance of the undiluted culture can be calculated by multiplying the absorbance reading of the diluted culture by the dilution factor. In this case, the dilution factor is 2 (total volume after dilution divided by initial volume), so the actual absorbance is 0.059 * 2 = 0.118.
I have to prepare a 1 to 10 dilution and have a 2ml culture suspension and want to prepare a 1 to 10 dilution of the entire culture.?
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
What is the technique you used in purify the bacterial culture on a plate?
I used streak plate technique to purify the bacterial culture on a plate. This involved streaking the culture onto the agar surface in a specific pattern to isolate individual colonies by dilution. Subsequent incubation allowed the colonies to grow separately, enabling the selection of pure cultures for further study.
Which approach to conflict management would be most appropriate for a culture that values collectivism?
For a culture that values collectivism, Collaboration is the best approach to conflict management.
What is a throat culture?
A throat culture is a technique for identifying disease bacteria in material taken from the throat.
The culture-general approach to cross-cultural competence PME?
Learning about culture and how it operates
Why is the streak plate preferred over the spot inoculations in this procedure?
The streak plate method is preferred over spot inoculations because it allows for the isolation of individual colonies from a mixed culture, promoting the separation of different microorganisms. This technique creates a gradient of dilution across the agar plate, enabling the growth of distinct colonies that can be easily identified and characterized. Additionally, the streak plate method minimizes the risk of contamination and provides a more systematic approach to isolating pure cultures.
Does the Culture Excellence Approach or Japanese Approach to management fall into post ford-ism theories?
yes
What is the correct technique to Introduce yourself?
Proper greetings vary from culture to culture. The proper greeting in American culture would be a handshake and an exchange of names.
What does an emic approach focus on?
what people say about their own culture.
How does the rivers of mesopotamia hurt the civilization?
It is possible that the rivers in Mesopotamia hurt the civilization by causing more people to move there thereby causing dilution of culture.
Which advance resulted from the development of a culture technique for muscle cells?
insulin production
