Diphenylamine is considered an internal indicator because it changes color in the presence of nitrates by forming a blue complex. This color change is used to detect the presence of nitrates in solutions without the need for an external indicator.
Sometimes one of the reactants in a titration act as an indicator; this is an internal indicator. An example is the volumetric technique of permanganometry.
Diphenylamine is a reagent for DNA.
Diphenylamine turns blue when it reacts with nitrate ions in the presence of sulfuric acid. This reaction forms a blue-colored complex called Ruhemann's purple. The intensity of the blue color can depend on the concentration of the reactants and the pH of the solution.
DNA
Diphenylamine turns blue in the presence of nitrates. This reaction is commonly used in the Griess test to detect the presence of nitrate ions.
Magnetite? Diphenylamine - http://pubs.acs.org/doi/abs/10.1021/ie50183a043
There are three indicators that may be used for the titration of Fe2+ with K2Cr2O7. These are diphenylamine, diphenylbenzidine and diphenylamine sulfonate. The colour change for all three indicators is green to violet and the standard electrode potentials are all ca 0.78 V. According to Kolthoff and Sandell, this should lie between the electrode potentials of the two reduction reactions. This not being the case, phosphoric acid is added to reduce the electrode potential for the Fe3+ → Fe2+ reaction by stabilising the ferric ion.
what is the difference between the external & internal indicator
Sometimes one of the reactants in a titration act as an indicator; this is an internal indicator. An example is the volumetric technique of permanganometry.
Diphenylamine is a reagent for DNA.
an indicator which is dissolved in the solution in whicdh the main reaction takes place- internal g. phenopthlein
The appearance of a blue color in the diphenylamine test indicates the presence of a reducing sugar, such as a reducing sugar or pentose sugar. The reaction occurs when the sugar present in the sample reduces diphenylamine to form a blue compound.
Diphenylamine turns blue when it reacts with nitrate ions in the presence of sulfuric acid. This reaction forms a blue-colored complex called Ruhemann's purple. The intensity of the blue color can depend on the concentration of the reactants and the pH of the solution.
what is the difference between the external & internal indicator
ferroin indicator
To prepare a nitrate test solution using diphenylamine in sulfuric acid, you can mix diphenylamine with concentrated sulfuric acid in a specific ratio. Typically, a 0.1% diphenylamine solution in concentrated sulfuric acid is used for nitrate testing. Follow safety precautions when working with concentrated sulfuric acid as it is corrosive.
DNA