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What is the role of DMEM in cell culture?
DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium that provides essential nutrients, vitamins, and minerals required for cell growth and proliferation. It helps maintain the pH and osmotic balance of the cell culture environment, supporting the growth of various cell types in vitro. DMEM can be supplemented with additional components such as fetal bovine serum, antibiotics, and growth factors to meet specific cell culture requirements.
Can sucrose be autoclaved?
Yes, sucrose can be autoclaved as it has a high thermal stability and does not readily decompose at typical autoclave temperatures (121°C). Autoclaving sucrose can effectively sterilize solutions or media containing it.
Can we autoclave SDS solution?
Yes, it is generally safe to autoclave an SDS (sodium dodecyl sulfate) solution. However, be cautious of potential foaming during the autoclaving process. It is advisable to use a proper container and allow sufficient headspace to prevent spills.
Do you put petri dishes in the autoclave?
Yes, petri dishes can be autoclaved to sterilize them. It is important to ensure that the petri dishes are made of materials that are autoclavable and that they are placed in appropriate autoclave-compatible containers before sterilization to prevent breakage.
Can you autoclave porcelain mortar?
Yes, porcelain mortars can be autoclaved. It is important to ensure that the mortar is heat-resistant and can withstand the high temperatures and pressure of the autoclave cycle to avoid damage. Properly clean the mortar before autoclaving to prevent any contamination.
How long do instruments remain sterile after being autoclaved?
Instruments remain sterile after being autoclaved for about 30 days if stored properly.
How does the presence of dmem glucose affect cellular metabolism in a laboratory setting?
In a laboratory setting, the presence of dmem glucose can affect cellular metabolism by providing a source of energy for the cells to use in various metabolic processes. Glucose is a key fuel for cells, and its presence can influence the rate at which cells grow, divide, and produce energy.
What is AACS?
If you concern for industries then AAC is Autoclaved Aerated Concrete.
What is the role of DMEM in cell culture?
DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium that provides essential nutrients, vitamins, and minerals required for cell growth and proliferation. It helps maintain the pH and osmotic balance of the cell culture environment, supporting the growth of various cell types in vitro. DMEM can be supplemented with additional components such as fetal bovine serum, antibiotics, and growth factors to meet specific cell culture requirements.
Would bleach sprayed on post autoclaved spunguard turn it black?
no
Unicon aerted concrete plant autoclaved batching plant in Kuwait?
Unicon AERTED CONCRETE PLANT â€" AUTOCLAVED (Batching Plant) â€" at KUWAIT, Manufacturing of cement tiles and cement blocks.
Can sucrose be autoclaved?
Yes, sucrose can be autoclaved as it has a high thermal stability and does not readily decompose at typical autoclave temperatures (121°C). Autoclaving sucrose can effectively sterilize solutions or media containing it.
How many days do items usually remain sterile after being autoclaved?
30 days
Can you pierce with acrylic jewelry?
You shouldn't, acrylic can have fissures and cracks that can harbour bacteria. Only proper surgical stainless steel or surgical titanium or Niobium should be used or a Bioplast or PTFE based material that can be autoclaved. Acrylics can not be autoclaved without damaging them.
Can polyethylene be autoclaved for sterilization purposes?
Yes, polyethylene can be autoclaved for sterilization purposes. Autoclaving is a common method used to sterilize medical equipment and materials by using high pressure steam. Polyethylene is a heat-resistant material that can withstand the high temperatures and pressures of the autoclave process without being damaged.
Growth media for streptomycin?
streptomycin is an antibiotic used in different types of cell culture media. Generally, this antbiotic is incorporated into DMEM (Delbucose Minimum Essential Medium) and used to culture mammalian cells
What is doubling time for HepG2?
48 h (DMEM containing 10% FBS, 5x104 cells/well in a 24-well plate); Wu Wen-Sheng, Oncogene (2003) 22, 955-963
