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Assuming you are asking about blue-white screening in transformation of plasmids...

The agar plate has X-gal in it. If a colony of E. coli has beta-galactosidase (an enzyme expressed from the lac operon in the vector) present, it will break down the x-gal and turn the colony blue. If the colony does not express beta-galactosidase (because the LacZ gene has been interrupted by a ligated gene that you want to express), it will not metabolize the x-gal, thus not turning blue.

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