answersLogoWhite

0

Assuming you are asking about blue-white screening in transformation of plasmids...

The agar plate has X-gal in it. If a colony of E. coli has beta-galactosidase (an enzyme expressed from the lac operon in the vector) present, it will break down the x-gal and turn the colony blue. If the colony does not express beta-galactosidase (because the LacZ gene has been interrupted by a ligated gene that you want to express), it will not metabolize the x-gal, thus not turning blue.

User Avatar

Wiki User

15y ago

What else can I help you with?

Related Questions

Where do red blood cells and white blood cells form?

Red and white blood cells produce in bones. The bone marrow produces the cells


What are some types of cells that have been transformed?

Some types of cells that have been transformed include cancer cells, stem cells, and bacterial cells for genetic engineering purposes. Transformation can involve altering the genetic material of a cell to express different characteristics or functions.


Does white blood cells have a large form?

Yes they do.


In which organ WBC's form?

The White Blood Cells form in the lymphoid organs.


What did Griffith find to be true after his experiments with the s and r strain of bacteria?

Griffith hypothesized that a chemical component from the virulent S cells had somehow transformed the R cells into the more virulent S form.


Is energy transformed or created?

Energy is transformed from one form to another. According to the law of conservation of energy, energy cannot be created or destroyed, only transformed from one form to another. For example, potential energy can be transformed into kinetic energy.


What is the definition of the word 'transformed'?

The definition of the word transformed is "To change markedly the appearance or form." A sentence that includes the word transformed is: "Transformed when incorporated into this concoction."


How do white blood cells form?

White blood cells form in response to an infection. As soon as the body detects a viral or bacterial infection, the immune system starts producing an army of white blood cells. They are produced in the bone marrow.


What is the medical terminology combining form meaning white?

Leuk- is the medical terminology combining form meaning white, as in leukocytes (white blood cells).leuko-leuk/o


Stem cells in human bone marrow divide and differentiate to form various what?

Stem cells in human bone marrow can divide and differentiate to form various types of blood cells, such as red blood cells, white blood cells, and platelets.


Can any form of energy be transformed to another form?

Yes.


Sugar's chemical bonds are transformed into a potent form of energy known as?

The chemical bonds of sugar are transformed into a potent form of energy known as ATP. It is responsible for transporting chemical energy within cells that will be used for metabolism.