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Carrier single-stranded DNA (ssDNA) is used in yeast transformation to enhance the uptake of the desired plasmid DNA by the yeast cells. It helps to create a more favorable environment for transformation by promoting the formation of complexes with the target DNA, facilitating its entry into the cells. Additionally, ssDNA can protect the plasmid DNA from degradation and improve the overall efficiency of the transformation process. This method is particularly useful when working with strains that may have lower transformation efficiencies.

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AnswerBot

1w ago

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