0
Once you have done chromotography and split up all of the amino acids in the protein you will need to measure from the spot where the sample was first placed to the top of the chromatography paper where the solvent stopped being drawn up through it. Next measure from the sample spot to the centre of each of the amino acid spots. Then, to calculate the Rf value divide the amino acid spot distance by the distance the solvent travelled. If you get an answer greater than one then you are dividing the numbers the wrong way round.
What else can I help you with?
What is the rf value of cocaine?
Rf is about .45
What is the Rf value of salicylamide?
It will depend on the solvent system you are using.
What is rf value and how is determine?
the mobility of any component in a particular solvent is referred to as its RF value. Mathematically, RF value = distance travelled by solute/ distance travelled by the solvent
What is the RF value for 4-aminophenol?
The Rf value for 4-aminophenol is the distance traveled by the compound divided by the distance traveled by the solvent front. The Rf value can vary depending on the specific solvent system and conditions used in the chromatography experiment.
What is Rf-value Why its has no unit?
There are no units because it is simply a ratio
What is the rf value of cocaine?
Rf is about .45
What is the Rf value of salicylamide?
It will depend on the solvent system you are using.
What is rf value and how is determine?
the mobility of any component in a particular solvent is referred to as its RF value. Mathematically, RF value = distance travelled by solute/ distance travelled by the solvent
Would you expect the Rf value of a pigment to be the same if a different solvent were used Explain?
The Rf value would not be the same for every solvent as there are factors that allow each solvent to be unique. The attractive force, particle size and solubility of each solvent will create different results each time.
What is an rf value used for?
The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.
What is the significance of the benzoic acid rf value in chromatography analysis?
The benzoic acid rf value in chromatography analysis is significant because it helps to identify and separate different compounds in a mixture based on their relative migration distances. By comparing the rf value of benzoic acid to other compounds, scientists can determine the purity and composition of a sample.
What is the maximum and RF value can be?
The maximum RF value that can be obtained is 1.0. This means that the substance travels the full distance of the chromatography medium being used. Any RF value greater than 1 is not physically possible.
How does saturation effect RF value?
In general, as saturation increases, the RF value decreases. This is because an increase in saturation tends to increase retention of the compound on the stationary phase, resulting in a lower RF value. Conversely, lower saturation levels may allow the compound to move more freely, leading to a higher RF value.
What is the RF value of benzoic acid in ethyl acetate?
The RF value of benzoic acid in ethyl acetate is 0.68.
What is the RF value for red ink blue ink and green ink?
the Rf value value is 8.44 as the particles are lighter than the solvent
What do the Rf values indicate about the relative solubility of the pigment in the the solvent?
The Rf values do not indicate the solubility of a substance. The Rf value or retardation factor is the ratio of the distance traveled by the center of a pot to the distance traveled by the solvent front in chromatography.
What is the relationship between chain length and Rf value?
As a general rule the longer the carbon chain the greater the Rf value.
