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Greater Prairie-Chickens are not threatened by severe winter weather. When the snow is thick they "dive" in to the snow to keep warm. A greater threat to the prairie-chickens comes in the from of spring rains. These sometimes drenching rains can wreak havoc on their chicks. Another major natural threat is drought. A drought can destroy food and make it difficult for the chicks.

Human interactions are by far the greatest threat. The conversion of native prairie to cropland is very detrimental to these birds. It was found in a radio telemetry study conducted by Kansas State University that "most prairie-chicken hens avoided nesting or rearing their broods within a quarter-mile of power lines and within a third-mile of improved roads." (Kansas Department of Wildlife and Parks) It was also found that the prairie-chickens avoided communication towers and rural farms.

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