The High powered field of view is harder to measure.
Low power magnifies to a smaller extent and has a wider field of view, while high power magnifies to a greater extent but has a smaller field of view.
If the field of view for high power is 300 microns, and a cell fills half of that field, then the approximate length of the cell would be around 150 microns. This is calculated by dividing the total field of view by 2.
The field for the high power objective is very small, so if you do not have the organism or whatever you want to view in focus on a lower power, it may be very difficult to find on high power. Also, the lens for the higher power objectives are longer than the lower ones, which makes them closer physically to the slide that you are viewing than the lower objectives. Thus you must use the fine tuning knob to sharpen the image in high power, because you could break the slide if you use the course adjustment knob.
A pus cell count of 2-4 per high power field (HPF) means there are 2 to 4 pus cells observed under the microscope in each high power field of the specimen. This count is a measure of the level of inflammation or infection present in the sample.
The diameter of the field of view decreases when changing from low to high power magnification. This is because higher magnification zooms in closer on the specimen, limiting the area of the specimen that can be seen at one time.
Low power magnifies to a smaller extent and has a wider field of view, while high power magnifies to a greater extent but has a smaller field of view.
As you move from low power to high power, the depth of field decreases. This means that at high power, the area in focus becomes smaller and more limited compared to low power. This is due to the increased magnification at high power which results in a shallower depth of field.
the diameter of the high power field microscope is 500 micrometers
When you change from low power to oil immersion high power on a microscope, the field of view decreases. This is because high power objectives have a narrower field of view due to higher magnification, leading to a more detailed but smaller area being visible through the lens.
If the field of view for high power is 300 microns, and a cell fills half of that field, then the approximate length of the cell would be around 150 microns. This is calculated by dividing the total field of view by 2.
The field for the high power objective is very small, so if you do not have the organism or whatever you want to view in focus on a lower power, it may be very difficult to find on high power. Also, the lens for the higher power objectives are longer than the lower ones, which makes them closer physically to the slide that you are viewing than the lower objectives. Thus you must use the fine tuning knob to sharpen the image in high power, because you could break the slide if you use the course adjustment knob.
NO, it means 10× high power field e.g. 1-2/10HPF means there are 1-2 per 10 × high power field
The reason it is more difficult is that the 'field of view' is much smaller, the higher the objective power. In other words, you don't have to be so exactly 'on target' with a lower objective power, as the field of view is larger in proportion to the object being viewed, so the object can be farther off to one side or the other, and still be seen. Once found in the lower power field, center the object in the field and then switch to the higher power- the object should then be in the higher power field of view. This is true for microscopes and telescopes, as well as any instrument that magnifies at different powers of magnification.
b/c the high power only allows you to see a small portion of thee slide. where as the low power pretty much allows you to see the whole slide all at once.
A pus cell count of 2-4 per high power field (HPF) means there are 2 to 4 pus cells observed under the microscope in each high power field of the specimen. This count is a measure of the level of inflammation or infection present in the sample.
The diameter of the field of view decreases when changing from low to high power magnification. This is because higher magnification zooms in closer on the specimen, limiting the area of the specimen that can be seen at one time.
Moving the letter e to the center of the low power field helps to ensure that it remains within the field of view when transitioning to high power. This adjustment prevents the specimen from being lost or moving out of sight when switching to a higher magnification.