It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
If the smear becomes too dense, it can hinder the staining process, making it difficult for dyes to penetrate and evenly stain the cells. This can lead to poor visibility of cellular structures and inaccurate results. To correct this, you should prepare a new slide with a thinner smear, ensuring a more even distribution of cells for optimal staining and observation.
Apply the crystal violet for one minute, flush with running water (gently), then apply Gram's iodine for one minute, flush gently with running water, immediately submerge in alcohol (perferably isopropyl) and gently agitate for about 10 seconds, gently flush with running water, and then apply a counter-stain (saffranin) for about 2 minutes, flush with running water gently and blot dry with bibulus paper.
It is best to remove the brown pollen tines from the lilies to prevent them from staining the petals or causing them to wilt prematurely. Gently pluck them off using your fingers or a pair of tweezers to keep the flowers looking fresh for longer.
E.coli is gram -ve and should stain pink in gram staining. The reason for its blue appearance is due to the over absorption of dye which can be due to either prolonged contact of dye or delay in washing or may be the concentration of soloution of dye is high
before use
You should typically wait at least 6 months before staining treated lumber to allow it to fully dry and settle.
Treated wood should dry for at least 6 months before staining to ensure proper adhesion and longevity of the stain.
A deck should typically dry for at least 48 hours before staining to ensure optimal results.
You should typically wait about 6 to 12 months before staining treated wood to allow it to fully dry and settle.
You should wait at least 6 months before staining pressure treated wood to allow it to fully dry and cure.
After sanding wood, it should be cleaned by wiping it down with a tack cloth or a damp cloth to remove any dust and debris before staining.
You should wait at least 6 months before staining pressure treated wood to allow it to dry and cure properly.
You should typically wait about 3-6 months before staining a new deck to allow the wood to properly dry and acclimate to its environment.
You should typically wait about 2-3 months before staining a new deck to allow the wood to fully dry and acclimate to its environment.
Pressure treated wood should typically dry for at least 6 months before staining to ensure proper adhesion and penetration of the stain.
A deck should typically dry for at least 24 to 48 hours before staining to ensure the wood is fully dry and ready to absorb the stain effectively.
Typically, one coat of pre-stain conditioner is sufficient before staining the wood.