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It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.

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What if the smear you are preparing for staining becomes to dense?

If the smear becomes too dense, it can hinder the staining process, making it difficult for dyes to penetrate and evenly stain the cells. This can lead to poor visibility of cellular structures and inaccurate results. To correct this, you should prepare a new slide with a thinner smear, ensuring a more even distribution of cells for optimal staining and observation.


In what time frame should gram staining be done?

Apply the crystal violet for one minute, flush with running water (gently), then apply Gram's iodine for one minute, flush gently with running water, immediately submerge in alcohol (perferably isopropyl) and gently agitate for about 10 seconds, gently flush with running water, and then apply a counter-stain (saffranin) for about 2 minutes, flush with running water gently and blot dry with bibulus paper.


IN MIDDLE OF lillies is brown pollen tines what should you do with them?

It is best to remove the brown pollen tines from the lilies to prevent them from staining the petals or causing them to wilt prematurely. Gently pluck them off using your fingers or a pair of tweezers to keep the flowers looking fresh for longer.


Why is EColi a gram negative bacteria?

E.coli is gram -ve and should stain pink in gram staining. The reason for its blue appearance is due to the over absorption of dye which can be due to either prolonged contact of dye or delay in washing or may be the concentration of soloution of dye is high


How often should backfire flame arrestors be inspected?

before use

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A deck should typically dry for at least 48 hours before staining to ensure optimal results.


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