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As you move your

loop

or wire across the

agar

you are removing

bacteria

from the loop. The reason it is essential is because every little bacterium that comes off that loop will grow like crazy on the agar, creating a colony. When you have an individual colony, after many times of streaking, you know, almost certainly, that you have genetically identical bacteria, barring mutations, without contamination.



So, simply put, to ensure your bacterial specimen is, to the best of your ability, without contamination.

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