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Isolation of any organelle requires a reliable test for the presence of the organelle. Typically, this is done by following the activity of an enzyme that is known to be localized exclusively in the target organelle. Such enzymes are known as marker enzymes. For example, the enzyme acid phosphatase (that cleaves terminal phosphate group from substrates and has a pH optimum in the

acidic range) is localized in lysosomes, while the enzyme succinate dehydrogenase is localized in mitochondria. By monitoring where each enzyme activity is found during a cell fractionationprotocol, one can monitor the fractionation of lysosomes and mitochondria, respectively.

Marker enzymes also provide information on the biochemical purity of the fractionated organelles. The presence of unwanted marker enzyme activity in the preparation indicates the level

of contamination by other organelles, while the degree of enrichment for the desired organelle is determined by the specific activity of the target marker enzyme. Although marker enzymes reveal much concerning the purity of the organelle preparation, electron microscopy is generally used as a

final step to assess the preparation's purity and the morphology of the isolated organelle.

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What would be a good enzyme lab for ib biology?

A good enzyme lab for IB Biology could involve investigating how factors such as temperature, substrate concentration, or pH affect the activity of an enzyme like amylase. Students could test the enzyme activity under different conditions using a spectrophotometer to measure changes in absorbance, or by observing changes in the rate of a reaction such as the breakdown of starch. This type of lab allows students to explore enzyme kinetics and understand the factors that influence enzyme activity.


Is adenine an enzyme?

No, adenine is not an enzyme. Adenine is one of the four nucleobases that make up DNA and RNA, while enzymes are proteins that catalyze biochemical reactions in living organisms.


What is found in allosteric enzymatic regulation?

An allosteric enzyme is one in which the activity of the enzyme can be controlled by the biniding of a molecule to the "allosteric site". This really just means somewhere other than the active site. Thus allosteric control of an enzyme can be classed in two ways. A positive allosteric modification is the binding of a molecule to the enzyme which increase the rate of reaction. Sort of like catalysing the catalysing effect of an enzyme. Obviously the opposite is true of negative allosteric modification. A good example for this is the activity of phosphofructokinase, which is promoted by a high AMP concentration, and inhibited by a high ATP concentration. This should make sense if you think about the action of a kinase etc.


Which component of a DNA synthesis reaction determines the exact nucleotide sequence of the product?

DNA polymerase. If it ends in -ase, odds are good that it's an enzyme.


How do you remove permanent marker from 95 percent cotton and 5 percent spandex?

One option is to try using rubbing alcohol or nail polish remover on a cotton swab to dab at the marker stain. Test in an inconspicuous area first to ensure it doesn't damage the fabric. Another option is to use a mixture of vinegar and dish soap to help break down the marker stain before washing the garment.

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Applications of chemotaxonomy include separation of higher systemic categories, and related genera having similar enzyme system producing analogous metabolites. It also includes complex metabolic products are good chemotaxonomic marker.


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Carrots, patatoes, and liver are good sources of enzyme catalase


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The imagine is too cheap of a marker to spend much on upgrades for, however a new barrel is always a good choice.


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Ok, considering the last person didn't give you a good answer, here's mine. The paintball "marker" is the proper term for the gun. It's called a marker because it "marks" what it shoots.


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A substrate is the substance acted upon by an enzyme. The enzyme substrate complex is when an enzyme molecule combines with its substrates.


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