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What type of media should be inoculated using zig-zag motion across the surface of the media?
Agar plates should be inoculated using a zig-zag motion across the surface of the media to evenly distribute the microbial sample and promote colony growth. This technique helps prevent overcrowding of colonies and facilitates the isolation of individual colonies for further analysis or study.
Why is MacConkey inoculated with a urine specimen?
MacConkey agar is selective for Gram-negative bacteria and can differentiate lactose fermenters from non-fermenters based on color change. Urine samples typically contain a mixture of bacteria, including potential pathogens that might be inhibited on other media. Inoculating MacConkey agar allows for the isolation and identification of Gram-negative bacteria, particularly Enterobacteriaceae, which are common urinary tract pathogens.
What are two ways to label your contrast media on the back table?
You can label contrast media by writing the patient's name and identification number on the label provided with the contrast media or by affixing a pre-printed sticker with the necessary information onto the contrast media container.
What type of media is used for growing a culture from a throat specimen?
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
Was life different before mass media?
Yes, life was different before mass media because people relied on word of mouth, written letters, and limited forms of communication to stay informed. Without mass media, information traveled more slowly and was often filtered through smaller, local sources. The advent of mass media revolutionized how information is disseminated and consumed on a global scale.
What type of media should be inoculated using zig-zag motion across the surface of the media?
Agar plates should be inoculated using a zig-zag motion across the surface of the media to evenly distribute the microbial sample and promote colony growth. This technique helps prevent overcrowding of colonies and facilitates the isolation of individual colonies for further analysis or study.
What are Mushroom Logs or Compost?
Mushroom Logs/Composts contains growing media/substrate inoculated with mushroom spawn. The white/brown material inside the bags is the "mycelium", which has "colonized" the growing media/substrate.
What is suspended between two binders in liquid media?
A microscope slide with a specimen mounted on it is typically suspended between two binders in liquid media for observation under a microscope. The liquid media helps maintain hydration and provides a suitable environment for viewing the specimen.
How do you inoculate a solid media?
To inoculate a solid media, a sterilized inoculation loop or needle is used to pick up a small amount of the desired culture. This culture is then streaked onto the surface of the solid media in a specific pattern to ensure isolated colonies grow. The inoculated media is then incubated at the appropriate temperature to allow the colonies to grow.
Why is MacConkey inoculated with a urine specimen?
MacConkey agar is selective for Gram-negative bacteria and can differentiate lactose fermenters from non-fermenters based on color change. Urine samples typically contain a mixture of bacteria, including potential pathogens that might be inhibited on other media. Inoculating MacConkey agar allows for the isolation and identification of Gram-negative bacteria, particularly Enterobacteriaceae, which are common urinary tract pathogens.
What is a mounting medium?
A mounting medium is a substance used in microscopy to secure the coverslip over a specimen on a slide. It helps to preserve the specimen, prevent distortion, and provide optical clarity for viewing under the microscope. Different types of mounting media can be used depending on the type of specimen and the desired preservation method.
Why should you use an inoculating needle when making smears form solid media?
You should use an inoculation needle for making smears from a solid media so you can control how much specimen is put onto a slide. This method is easier for solid media and you would use a loop for liquid media.
Prewarm agar plates?
To prewarm agar plates, simply place them in a 37°C incubator for about 30 minutes before use. This ensures that the agar solidifies evenly and prevents condensation from forming on the plates when they are inoculated. Always handle prewarmed plates carefully to maintain sterility.
When is irrigation for impacted cerumen contraindicated?
Irrigation should not be used to remove cerumen if the patient's eardrum is ruptured or missing; if the patient has a history of chronic otitis media.
What are two ways to label your contrast media on the back table?
You can label contrast media by writing the patient's name and identification number on the label provided with the contrast media or by affixing a pre-printed sticker with the necessary information onto the contrast media container.
How can an air bubbles are trapped under the specimen?
Air bubbles can get trapped under a specimen during mounting or preparation due to incomplete removal of air or improper sealing of the edges. This can lead to artifacts and distortions in the specimen image during microscopy. To prevent this, it's important to ensure proper cleaning and mounting techniques, as well as using appropriate mounting media to minimize air entrapment.
Who owns the information contained within medical records?
The patient owns the information; the doctor owns the media or paper.
