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Why is the streak-stab technique preferred rather than incubating the plates anaerobically?
The streak-stab technique is preferred over incubating the plates anaerobically because when isolating colonies allows biochemical testing to be performed. When the plate is incubated anaerobically it lacks oxygen and can not be biochemically tested.
What explanation could be given for the failure of obtaining isolated coloni es on a streak plate?
Failure to obtain isolated colonies on a streak plate could be due to overcrowding on the plate, improper streaking technique, or contamination of the plate from the environment or the inoculation source. It is important to streak the plate in a way that allows for sufficient separation of individual colonies to form.
Why must we use sterile techniques to incubate agar plates?
Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.
What are the 9 major plates and 8 minor plates?
There are many Tectonic plates on Earth. There is the African Plate, Antarctic plate, Eurasian Plate, Indo Australian plate, North American Plate, Pacific plate, South African Plate, Arabian Plate, Caribbean Plate, Cocos Plate, Indian Plate, and Juan de Fuca Plate.
What plates was involved in the japan earthquake?
thee ceramic plate, and the metal plate :)
Why do count plates with 20-200 colonies in the spread plate technique?
Well UC Student, counting a plate between 20 and 200 colonies provides an accurate estimation of the total number of bacteria. Counting plates with less than 20 colonies would providen unreliable data, while plates with 200 or more colonies is too difficult to do with the human eye. Don't be scared to ask you tutor questions, A-UNIT
Why are colonies on a heavily seeded plate smaller than those that appear on a sparsely seeded plate?
Microbe colonies develop in larger sizes on sparsely seeded plates due to the abundance of plate surface they have for growth. Heavily seeded plates produce smaller colonies as they are forced to compete with one another for basic survival.
What does the streak plate method have over the pour plate method?
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
How does the contribution of growth on a pour plate different from that on a steak plate?
Colonies growing on a pour plate have slightly less avalible oxygen and are confined by the gel matrix so they tend to grow smaller than those on a pour plate. Streak plates are use to isolate single colonies, pour plates are used to enumerate batceria.
How do the colonies on the surface of the pour plates differ from those suspended in the agar?
How do colonies on the surface of a pour plate differ from those suspended in the agar?
The technique in which bacteria from all of the colonies of one plate are simultaneously trasnferred to the same position on another plate is called?
Replica plating method.
What is the differences between a streak plate technique and a serial dilution technique?
A streak plate technique is used to isolate individual bacterial colonies on a solid agar plate to obtain pure cultures, while a serial dilution technique is used to dilute a bacterial sample in a series of steps to obtain a range of concentrations for further analysis. Streak plate technique is qualitative, focusing on colony isolation, while serial dilution technique is quantitative, focusing on estimating bacterial concentration.
Why is the streak-stab technique preferred rather than incubating the plates anaerobically?
The streak-stab technique is preferred over incubating the plates anaerobically because when isolating colonies allows biochemical testing to be performed. When the plate is incubated anaerobically it lacks oxygen and can not be biochemically tested.
What is the technique you used in purify the bacterial culture on a plate?
I used streak plate technique to purify the bacterial culture on a plate. This involved streaking the culture onto the agar surface in a specific pattern to isolate individual colonies by dilution. Subsequent incubation allowed the colonies to grow separately, enabling the selection of pure cultures for further study.
How would one define the word etch?
Etching is a technique for drawing pictures into glass or metal plates. In this technique, cuts are made on the plate along the desired lines with weak acids.
What explanation could be given for the failure of obtaining isolated coloni es on a streak plate?
Failure to obtain isolated colonies on a streak plate could be due to overcrowding on the plate, improper streaking technique, or contamination of the plate from the environment or the inoculation source. It is important to streak the plate in a way that allows for sufficient separation of individual colonies to form.
Why must we use sterile techniques to incubate agar plates?
Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.
