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What else can I help you with?
What is the influence of the pH on the gram reaction in gram staining?
In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process. If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
How do you fix a microbiology slide?
In order to heat fix your microbe to your slide - you need to let your smear air dry. Once it is completely air dried. Pass the slide 2 or 3 times quickly over an open flame like a Bunsen burner. This does three things, 1. Kills the bacteria 2. Firmly affixes the smear to the microscope slide. 3. Allows the sample to more readily take up the stain.
Why use methanol to fix smear during giemsa staining?
because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.
Microbiology How does the charge on a Microorganism affect staining Which staining protocols rely on which charges What are the outcomes?
The charge of the microorganism is usually negative. The cell membrane is a phospholipid bilayer which is polar, the outside is usually negative. Depending on the staining protocol, a dye will be repelled by or attracted to the cell. Sometimes you use two different dyes- one to color the cell and then one to color the background or surrounding area. Sometimes you want to color the inside of the cell, so you need to melt the cell membrane or cell wall away with heat or chemicals. This allows the dye back in. Sometimes there are steps to resolidify this barrier to trap the dye inside prior to rinsing the slide with water or whatever. These steps are tricky and easy to screw up completely, resulting in a clear or invisible specimen because the dye didn't "take".
Do I need to apply wood conditioner before staining?
Yes, it is recommended to apply wood conditioner before staining to help the wood absorb the stain more evenly and prevent blotchiness.
Do you need to apply wood conditioner before staining?
Yes, it is recommended to apply wood conditioner before staining to help the wood absorb the stain more evenly and prevent blotchiness.
What is the influence of the pH on the gram reaction in gram staining?
In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process. If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.
Do I need to condition wood before staining it?
Yes, it is recommended to condition wood before staining it to ensure an even and smooth finish. Conditioning helps the wood absorb the stain more evenly and reduces blotching.
Do you need to condition wood before staining it?
Yes, it is recommended to condition wood before staining it to ensure an even and smooth finish. Conditioning helps the wood absorb the stain more evenly and prevents blotching.
What requires more light a stained specimen slide or an unstained live specimen slide?
A stained specimen slide typically requires more light than an unstained live specimen slide. This is because the staining process can reduce the transparency of the specimen, making it harder for light to pass through and creating a need for more illumination to visualize details.
How long does a deck need to dry before staining it?
A deck typically needs to dry for at least 24 to 48 hours before staining it. It is important to ensure that the wood is completely dry to allow the stain to adhere properly and provide a long-lasting finish.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
What equipment should be used when making slides of cheek cells to observe under a microscope?
To make slides of cheek cells for microscopy, you will need a microscope slide, cover slip, swab or toothpick to collect the cells, a staining solution (such as iodine), and a microscope with at least 400x magnification. You may also need a pipette or dropper to apply the staining solution.
How do you fix a microbiology slide?
In order to heat fix your microbe to your slide - you need to let your smear air dry. Once it is completely air dried. Pass the slide 2 or 3 times quickly over an open flame like a Bunsen burner. This does three things, 1. Kills the bacteria 2. Firmly affixes the smear to the microscope slide. 3. Allows the sample to more readily take up the stain.
How long do you need to wait before you can stain treated lumber?
You should wait at least 6 to 12 months before staining treated lumber to allow it to fully dry and settle.
What happens when you eat fatty foods before intake of alcohol?
You need much more alcohol to get drunk. It is most cost effective to drink before (or instead of) eating. Consumption of fatty meals after excessive alcohol intake does not generally annul the effects of alcohol.
