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Why different component different number of theoretical plates?

Different component may have different retension time attached to stationary phase and size.


Why the sample need dilution?

Dilution is done to decrease the amount of colonies found in the plates. If the solution is not diluted, it would be difficult to count the number of colonies because they would all overlap one another. So by dilution, we can limit the amount of colonies overlapping each other, count them, and used this number to estimate the number of bacteria in the original sample.


What are the problems with agar plates?

Agar plates can become contaminated with unwanted microorganisms, leading to inaccurate results. They can also dry out, affecting the growth of bacteria. Additionally, agar plates can become overcrowded with bacterial colonies, making it difficult to differentiate and count individual colonies.


Why are plates with 25 to 250 colonies used for calculations?

Well UC Student, counting a plate between 20 and 200 colonies provides an accurate estimation of the total number of bacteria. Counting plates with less than 20 colonies would providen unreliable data, while plates with 200 or more colonies is too difficult to do with the human eye. Don't be scared to ask you tutor questions, A-UNIT


How do you count colonies on agar plates?

To count colonies on agar plates, first ensure the plates are incubated properly and are at room temperature. Use a colony counter or a marker to outline each colony, counting them systematically. It's important to only count distinct, well-isolated colonies and to avoid counting any that are too close together or appear to be clumped. For accuracy, record the counts for multiple plates and calculate an average if necessary.

Related Questions

What observations can you make about the variety and number of colonies observed on the different plates?

The number and variety of colonies observed on the plates can provide insights into the diversity and abundance of microorganisms present in the sample. A higher number of colonies indicates a more diverse microbial community, while different colony morphologies suggest the presence of multiple bacterial species with unique characteristics. Variation in colony size, shape, color, and texture can also indicate different types of microorganisms present in the sample.


Why are number plates different colors?

Number plates come in many different colours and types. This is not surprising because different countries and different states within countries design their own number plates. Within the same state or country, sometimes number plates are differently coloured to represent a type of vehicle (for example, a truck, taxi, or government vehicle), or because they are special "vanity plates" that you can customise for a fee.


What are the rules regarding show and legal number plates?

The DVLA in the Uk carry the specific up to date legal requirements for number plates the US and other countries have their own sources


What does the streak plate method have over the pour plate method?

It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.


Where can someone purchase plastic camping gear such as plates cups or bowls online?

You can purchase plastic camping gear such as plates, cups or bowls from various different sources online. These sources include but are not limited to retailers such as Amazon and eBay.


Why do count plates with 20-200 colonies in the spread plate technique?

Well UC Student, counting a plate between 20 and 200 colonies provides an accurate estimation of the total number of bacteria. Counting plates with less than 20 colonies would providen unreliable data, while plates with 200 or more colonies is too difficult to do with the human eye. Don't be scared to ask you tutor questions, A-UNIT


Where could one purchase fine white china dinner plates?

A person could purchase white china dinner plates through a variety of different sources. Some of the more popular sources are Macy's, Bed Bath & Beyond and Amazon.


How many different license plates can be formed if each one consists of 7 digit number and each number can occur once and the first number can't be a zero?

The number of different 7 digit plates that can be formed where the digits are notrepeated and no zero appears as the first digit is: 544 320.Calculation:Number of different plates = 9 [9P6] = 9 [9!/(9-6)!] = 9x9x8x7x6x5x4 = 544 320.


Why different component different number of theoretical plates?

Different component may have different retension time attached to stationary phase and size.


Why are number plates called number plates if they have letters in them?

We call them license plates where I live.


Why the sample need dilution?

Dilution is done to decrease the amount of colonies found in the plates. If the solution is not diluted, it would be difficult to count the number of colonies because they would all overlap one another. So by dilution, we can limit the amount of colonies overlapping each other, count them, and used this number to estimate the number of bacteria in the original sample.


When do the new number plates for cars come?

The new plates come in at different times. Some states comes in annually and some many years apart.