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M.O are always present,the air, on laboratory surfaces, benches and equipment.they can serve as a source of external contamination and thus interfere with experiment results unless proper aseptic techniques are used during subculturing.

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Why heat the inoculating loop after each transfer of microorganisms?

It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.


Why is aseptic technique necessary for successful cultivation of pure cultures in the laboratory?

Short answer is Koch's postulates. Long answer you can't ascribe any scientific data you may gather to a bunch of bacteria on a plate or in a culture, as you won't know which of the bacteria gave you the result you observed. So if you are trying to figure out which bacteria causes a disease, inoculating a mix of bacteria won't tell you which one causes the disease. Isolating each one using aseptic technique and then inoculating individually and finding the disease in one animal will tell you which bacteria caused to disease.


Why must make 4 quadrant of streak plate method?

The four-quadrant streak plate method is used to isolate individual bacterial colonies from a mixed culture. By dividing the agar plate into four sections and systematically streaking each quadrant with a sterilized inoculating loop, the microbial density decreases with each streak. This technique allows for the separation of distinct colonies, making it easier to identify and study specific microorganisms. Ultimately, it enhances the accuracy of isolating pure cultures for further analysis.


After mitosis each identical daughter cell has a complete set of what?

DNA replicates before a cell divides ensures that each daughter cell has a complete set of genetic material They must have an identical set of DNA.


What is the charge of each atom before forming the ionic bond?

Before the forming of the bond atoms are neutral; after the forming of the bond atoms become cations or anions.

Related Questions

What are the other inoculating instruments aside from needle?

Some other inoculating instruments include loops, spreaders, swabs, and pipettes. These tools are used to transfer and spread microorganisms onto various growth media for cultivation and observation in laboratory settings.


Why heat the inoculating loop after each transfer of microorganisms?

It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.


What is the reason for flaming the tubes before and after each transfer?

Flaming the tubes before and after each transfer is a crucial practice in microbiological techniques to prevent contamination. The heat creates a sterile environment by killing any potential microbes on the lip of the tube, ensuring that only the intended microorganisms are transferred. This helps maintain the integrity of the culture and ensures accurate experimental results. Additionally, it serves as a visual signal that the tube has been properly sterilized.


Who is the 20th century leader introduced the custom of carrying a flaming torch from Athens to the site of each olympic games?

jad osta


In subculturing when do you use the inoculating loop?

Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.


What is the name of the song used in the new dell laptop commercial where each person has their own colored laptop?

It's not called The W.A.N.D. by the Flaming Lips that's wrong


1 how are microorganisms diluted and spread out to form individual colonies In the streak plate technique?

Bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and then streaked out in one of several patterns. At some point, individual cells will be removed from the loop and will give rise to separate colonies.source: http://quizlet.com/17578430/micro-lab-unit-1ex-15-16-streak-plate-technique-flash-cards/


How many rocks fall into the Styx on Mythology Island?

There are usually 3 sets of hazards on the River Styx, each set consisting of one rock fall, one flaming skull, and one river monster.


How many rounds do you have to go through to cross the river on Mythology Island?

In Hades Realm on the River Styx, each set of hazards has a rock, a flaming skull, and a river monster. Usually there are 4 sets but there can be 5 sets.


What did Wayne Gretzky eat before each game?

Wayne Gretzky ate two hotdogs before each game I believe.


What type of strategies can educators use to ensure their students are not flaming in way that causes damage to others?

They can use one on one teaching to assist the student. They can also give out special assignments to each student to help assist with their learning styles.


How many chromatids does each chromosome have before S-phase?

There are two chromatids for each chromosome before the S-phase. The S-phase is wherein DNA of each chromosome is replicated.