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Is there a molar mass of 4.0 PH buffer?

Ph gives the hint about the proton gradient of the solution as pH=-log[H+] we need the inforamtion of the molecular composition of the solution to know about the molar mass. Ph gives the hint about the proton gradient of the solution as pH=-log[H+] we need the inforamtion of the molecular composition of the solution to know about the molar mass.


What is the need for an ammonical buffer solution in the determination of hardness of water by edta titration?

during the complexometric titration using edta it is very necessary to maintain the ph of the solution near about 10 so we use ammonium chloride buffer if we will not use this buffer dring the titration ph of sol. will ho lower side


What is the buffer solution and why is it added to the agarose?

You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good


Why is a buffer solution need for electrophoresis?

Electrophoresis refers to the movement of charged particles in a fluid or gel under the influence of an electric field. It is carried out in buffer in order to complete the electron circuit flow.


Is there any calculation to make phosphate buffer at pH 7?

Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html

Related Questions

Is there a molar mass of 4.0 PH buffer?

Ph gives the hint about the proton gradient of the solution as pH=-log[H+] we need the inforamtion of the molecular composition of the solution to know about the molar mass. Ph gives the hint about the proton gradient of the solution as pH=-log[H+] we need the inforamtion of the molecular composition of the solution to know about the molar mass.


How do you prepare 50mM TES buffer?

In order to prepare 50mM TES buffer, you will need to add in approximately 1000 ml of Proteinase K solution. From there, you will need to separate and stack the gels.


What materials do you need for PCR?

For PCR, you will need DNA sample, primers, nucleotides, DNA polymerase, buffer solution, and a thermal cycler.


How much 50x TAE buffer will it take to make 3L TAE solution?

To prepare a 3L (3000 mL) TAE solution using 50x TAE buffer, you would need to dilute the 50x buffer by a factor of 50. Therefore, you would take 60 mL of the 50x TAE buffer and add it to 2940 mL of distilled water to achieve a final volume of 3L of 1x TAE solution.


How do you calculate the composition of a buffer solution?

To calculate the composition of a buffer solution, you need to consider the concentrations of the weak acid and its conjugate base. The Henderson-Hasselbalch equation is commonly used for this calculation, which is pH pKa log(A-/HA), where A- is the concentration of the conjugate base and HA is the concentration of the weak acid. By knowing the pH, pKa, and concentrations of the acid and its conjugate base, you can determine the composition of the buffer solution.


I need help on figuring out the total ionic and net ionic equations for adding an acid (CH₃COOH) to a buffer solution ofNaHCO3 + Na2CO3 and also adding a base (NH₃) to a buffer solution of NaHCO3 + Na2CO3?

don't mes with that stuff its very dangerous.


How do you buffer a solution that has the pH of 12?

To buffer a solution with a pH of 12, you would need to add a weak acid and its conjugate base in appropriate amounts. This will help prevent drastic changes in pH when an acid or base is added to the solution. Common buffer systems for a pH of 12 could involve using a weak base like ammonia (NH3) and its conjugate acid ammonium chloride (NH4Cl).


How do you calculate a buffer solution?

To calculate a buffer solution, you need to determine the amount of a weak acid and its conjugate base needed to maintain a stable pH. This is done by using the Henderson-Hasselbalch equation, which involves the concentrations of the acid and its conjugate base, as well as the pKa of the acid.


How you standardize pH meter?

Standardize the pH meter using a buffer solution of known pH value.Basically take buffer of pH value 4.Then set zero reading in the pH meter.Now remove unknown buffer solution.(take care with atmospheric temperature.)


How do you perform buffer calculations effectively?

To perform buffer calculations effectively, you need to first determine the pH range needed for your solution. Then, choose a weak acid and its conjugate base with a pKa close to your desired pH. Calculate the amount of each component needed using the Henderson-Hasselbalch equation. Finally, mix the components in the correct proportions to create the buffer solution.


What is the need for an ammonical buffer solution in the determination of hardness of water by edta titration?

during the complexometric titration using edta it is very necessary to maintain the ph of the solution near about 10 so we use ammonium chloride buffer if we will not use this buffer dring the titration ph of sol. will ho lower side


Why do you add iron III chloride buffer to the salicylate solution?

Iron III chloride is added as a reagent to form a colored complex with salicylate ions. This complex has a strong absorbance at a specific wavelength, allowing for the accurate detection and quantification of salicylate in the solution. The buffer helps maintain the pH of the solution, ensuring a stable environment for the formation of the complex.