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Definition

A buccal smear (pronounced "buckle") is the painless removal of a sample of cells from the lining of the mouth (inside of the cheek) for study.

Alternative Names

Sex chromatin test

How the test is performed

The health care provider will gently scrape the inside of the cheek with a small spatula to collect cells for testing.

How to prepare for the test

Rinse and wash the mouth as instructed.

How the test will feel

You will feel a scraping sensation as cells are removed from the cheek.

Why the test is performed

The procedure is used to obtain cells for chromosome and DNA analysis,most often for genetic testing. The International Olympic Committee adopted the test many years ago to help detect male imposters among female athletes. When the test is used in this way, it's called the sex chromatin test. This method may also help establish the sexual identity of newborns.

What the risks are

There are no risks.

Reviewed By

Review Date: 12/15/2010

Frank A. Greco, M.D., Ph.D., Director, Biophysical Laboratory, The Lahey Clinic, Burlington, MA. Review provided by VeriMed Healthcare Network. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.

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What is the steps for performing a cheek cell (buccal) in the correct order?

To perform a cheek cell (buccal) smear, start by rinsing your mouth with water and spitting it out to remove debris. Next, use a sterile swab to gently scrape the inside of your cheek to collect epithelial cells. Then, smear the collected cells onto a glass microscope slide to create a thin layer. Finally, allow the slide to air dry, and optionally, fix and stain the sample for microscopic examination.


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The area of a blood smear that is tested is called the peripheral blood smear. It is a sample of blood that is spread thinly on a glass slide for examination under a microscope to evaluate the number, shape, and size of blood cells.


Give the reason for passing bacterial smear through the flame before staining?

Passing the bacterial smear through the flame before staining is done to heat-fix the bacteria onto the slide, making them adhere firmly and preventing them from washing off during the staining process. Heat fixing also kills the bacteria, which helps in the preservation of their cellular structures for visualization under the microscope.


What is the most common shape of bacteria seen in a typical cheek smear?

The most common shape of bacteria seen in a typical cheek smear is cocci, which are spherical or round in shape.


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Related Questions

What is the steps for performing a cheek cell (buccal) in the correct order?

To perform a cheek cell (buccal) smear, start by rinsing your mouth with water and spitting it out to remove debris. Next, use a sterile swab to gently scrape the inside of your cheek to collect epithelial cells. Then, smear the collected cells onto a glass microscope slide to create a thin layer. Finally, allow the slide to air dry, and optionally, fix and stain the sample for microscopic examination.


Why do you do buccal smear testing for barr bodies?

Buccal smear testing for Barr bodies is performed to determine the presence of an extra X chromosome in females, which indicates a condition such as Turner syndrome or Klinefelter syndrome in males. This test is a non-invasive method that allows for the visualization of the Barr body, which is the inactivated X chromosome, by collecting cells from the inside of the cheek. It is useful in clinical genetics for diagnosing chromosomal abnormalities and understanding sex chromosome disorders. Additionally, it can be a helpful tool in prenatal diagnosis and research on sex differentiation.


Can a pap smear stop you from conceiving?

A pap smear does not affect your fertility. You are as likely to get pregnant after a pap smear as you are before a pap smear.


What can be observed on a plaque smear wet mount?

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What is the abbreviation for papanicolaou smear?

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The past tense of smear is smeared.


Can you give me a sentence using the word smear?

The whole scandal is nothing more than a rival smear campaign.We will need to perform a smear test.She began to smear the paint on the canvas with her bare hands.


Why do you air dry a smear?

To avoid denaturing and destroying the smear.


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Steve Smear was born in 1948.


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Pop Smear was created in 1993.


How many syllables does smear have?

Smear is monosyllabic.


When was Pat Smear born?

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