Brain natriutetic peptide (BNP) test is a blood test that measures levels of a protein made by your heart and blood vessels. This protein is called BNP. BNP levels are higher than normal when you have heart failure.
How the test is performedA blood sample is needed. For information on how this is done, see: Venipuncture
This test is usually done in the emergency room or hospital. Results take up to 15 minutes. In some hospitals, a finger prick test with rapid results is available.
How the test will feelWhen the needle is inserted to draw blood, you may feel moderate pain, though most people feel only a prick or a stinging sensation. Afterward there may be some throbbing or bruising.
Why the test is performedYour doctor may order this test if you have signs of heart failure. Symptoms include shortness of breath and swelling of your legs or abdomen. The test helps your doctor make sure the problems are due to your heart and not your lungs, kidneys, or liver.
It is unclear if repeated BNP tests are helpful in guiding treatment in those already diagnosed with heart failure.
Normal ValuesIn general, results less than 100pg/mL are a sign of heart failure.
The examples above are common measurements for results of these tests. Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.
What abnormal results meanBNP levels go up when the heart cannot pump the way it should.
A result greater than 100 pg/mL is abnormal. The higher the number, the more likely heart failure is present and the more severe it is.
What the risks areThere is very little risk involved with having your blood taken. Veins and arteries vary in size from one patient to another and from one side of the body to the other. Taking blood from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight but may include:
A related test, called the N-terminal pro-BNP test, is done in the same way. It provides similar information to your health care provider.
ReferencesRedfield MM. Heart Failure with normal ejection fraction. In: Bonow RO, Mann DL, Zipes DP, Libby P, eds. Braunwald's Heart Disease: A Textbook of Cardiovascular Medicine. 9th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 30.
Jessup M, Abraham WT, Casey DE, Feldman AM, Francis GS, Ganiats TG, et al. 2009 focused update: ACCF/AHA Guidelines for the Diagnosis and Management of Heart Failure in Adults: a report of the American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines: developed in collaboration with the International Society for Heart and Lung Transplantation. Circulation. 2009 Apr 14;119(14):1977-2016.
Lindenfeld J, Albert NM, Boehmer JP, et al. Executive Summary: HFSA 2010 Comprehensive Heart Failure Practice Guideline. J Card Fail 2010;16:475e539.
Reviewed ByReview Date: 10/13/2011
David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc., and Michael A. Chen, MD, PhD, Assistant Professor of Medicine, Division of Cardiology, Harborview Medical Center, University of Washington Medical School, Seattle, Washington.
BNP is a blood test.
in biuret test the presence of peptide bonds gives a violet color. but it should be noted that a minimum of two peptide bonds should be present in the protein to get a positive answer to this test.
the results are negative because pesin breaks peptide bonds. therefore no peptide will be found in the test.
You must have at least two, those that will form the peptide linkage (NH2 and COOH) since Biuret test is for detecting peptide linkages.
No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.
B-type natrurietic peptide. 
Casein tests positive in the Biuret test because it is a protein that contains peptide bonds. The Biuret test detects the presence of these peptide bonds by forming a violet color when copper ions in the reagent interact with them. Since casein is composed of long chains of amino acids linked by peptide bonds, it readily produces a positive result in this test.
The B-type Natriuretic Peptide test, is a blood test which is looking for any type of heart failure. The level of BNP increases when there are symptoms of heart failure.
The biuret test is a chemical test used for detecting the presence of peptide bonds. In a positive test, a copper(II) ion is reduced to copper(I), which forms a complex with the nitrogens and carbons of the peptide bonds in an alkaline solution. A violet color indicates the presence of proteins.It is possible to use the Biuret reaction to determine the concentration of proteins because (for most proteins) peptide bonds occur with approximately the same frequency per gram of material. The intensity of the color, and hence the absorption at 540 nm, is directly proportional to the protein concentration, according to the Beer-Lambert law.
At least two peptide bonds must be present in a molecule to give a positive biuret test. The biuret reagent interacts with peptide bonds in proteins, forming a colored complex that can be detected visually.
The biuret test can be used to show the hydrolysis of proteins. In this test, a blue to purple color change indicates the presence of peptide bonds being hydrolyzed. This color change occurs due to the formation of a coordination complex between copper ions and the peptide bonds.
Yes. The Biuret test is a test for the presence of peptide bonds. Since albumin is a class of protein, and proteins are composed of many amino acids joined together by peptide bonds, the test will yield a positive result.