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Yes, but only after Duncan's murder. As Macbeth thought he heard, "Macbeth shall sleep no more," because he murdered sleep. He murdered Duncan in his sleep and his guilt murders his sleep. Lady Macbeth is also effected by all of this. Later in the play, she sleep walks and tries to wash the blood off of her hands, which symbolizes her unending guilt. She is reliving the nights that Duncan and Banquo were murdered and she is so paranoid that she must sleep with a candle so that she is not murdered in her sleep like Duncan. She is so guilty that she eventually commits suicide.

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