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UV light causes the compounds on a TLC plate to absorb the light, which excites the electrons in the molecules to a higher energy state. When the electrons return to their original state, they release energy in the form of fluorescence and produce visible spots on the TLC plate.
What else can I help you with?
What would cause the TLC light to come on?
Never heard of a TLC light on a car.
When preparing to develop a TLC plate what is the appropriate spatial relationship between the level of solvent in the developing chamber and the spots on your TLC plate?
The spots on the TLC plate should be placed about a cm from the bottom of the plate. Then solvent should then fall about half-way between these spots and the bottom of the beaker.
Do you appear in the Undertaker TLC match2011 or not?
no
What will be the result of applying too much compound to a TLC plate?
Applying too much compound to the TLC plate makes it difficult for a good separation to occur. Instead, a large blot of solution tends to appear in the TLC. "It is extremely important that the spots be as small as possible and that they be applied using a 1% solution of the compounds being separated
What would be the effect when the solvent pool in a developing jar is too deep in TLC technique?
If the solvent pool in a TLC developing jar is too deep, it can cause the sample spots to become diffused or spread out. This can lead to poor resolution and overlapping of the compounds being separated on the TLC plate. It may also make it difficult to accurately analyze and interpret the results.
What TV channel is TLC in Charleston South Carolina?
TLC - The Learning Channel does not appear to be available in the Charleston, SC, viewing area.
1. What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development?
If a solvent of too low polarity is used for the development of a TLC plate, the substances may not migrate effectively, resulting in little to no separation. The spots of the compounds may remain close to the baseline, or they could appear as faint streaks. Overall, the TLC plate will show minimal movement of the compounds, indicating that the solvent was insufficiently polar to facilitate their migration.
What happen if the spots on TLC plate are made too small?
There will be too much of the sample for it to effectively separate over such a short distance. The result will be a splotchy streak instead of several separate spots after development.
Why can iodine be used in the visualization of TLC spots?
The majority of compounds adsorb iodine and become visible if exposed to iodine vapor. Although reaction may take place with some unsaturated compounds, for the vast majority of compounds, the iodine appears to be physically bound to the material and does not chemically react. The after carrying out the elution process the plate is dried and placed in an enclosure containing iodine crystals and is preferably heated to about 50oC. Brown spots appear where the solutes are situated. When the plate is removed from the enclosure, the spots rapidly disappear and so this detection technique appears to be essentially non-destructive. This detection procedure can be improved by first exposing the plate to a very high concentration of iodine vapor, the plate is the removed and allowed to stand for a few minutes to allow the excess iodine to evaporate. The plate is then sprayed with a 1% starch solution. As a result the solute bands appear as blue spots on the plate. These methods will detect most organic substances and in particular unsaturated compounds.
Why should a TLC plate be removed fromthe solvent before the solventfront reaches the top of the plate?
Removing the TLC plate before the solvent front reaches the top of the plate helps prevent the sample from oversaturating and distorting the spots. It also ensures that the solvent does not evaporate completely, which could cause the stationary phase to dry out and affect separation results.
What channel has 'Twist Of Kate'?
The Twist of Kate will appear on TLC channel 34 but the date is unknown
What techniques are used to identify spots on a chromatogram?
Techniques used to identify spots on a chromatogram include comparing the retention factor (Rf) values of the spots with known standards, visualizing spots using UV light or specific staining reagents, and analyzing the spots through mass spectrometry or infrared spectroscopy. Additionally, methods such as thin-layer chromatography (TLC) often employ mobile phase solvents to separate components, allowing for clearer identification. These techniques help determine the identity and concentration of compounds in the sample.
