A blue filter only allows shorter wavelengths of light to pass. So, covering the light source of a light microscope with a blue filter shortens the wavelength of light passing through the objective.
Yes, it is possible to shorten a wavelength by increasing the frequency of the wave. This relationship is described by the wave equation λ = c/f, where λ is wavelength, c is the speed of light, and f is frequency.
Viruses. They are smaller than the average wavelength of light and as such are not viewable unless an electron microscope is used.
When you shorten the wavelength, the energy increases. This is because energy is inversely proportional to wavelength according to the equation E = hc/λ, where E is energy, h is Planck's constant, c is the speed of light, and λ is the wavelength. Shortening the wavelength increases the frequency, thus increasing the energy of the wave.
Viruses are too small to be seen with a light microscope because they are smaller than the wavelength of visible light. This means that light cannot pass through them or be reflected off of them, making them invisible under a light microscope.
== == When using a light microscope you encounter diffraction. (Visible light behaves like a wave, with a wavelength of about 300 to 900 nanometres). We say that light diffracts when its wavelike behaviour makes it bend around obstacles, or spread out. If the obstacle is much larger than the wavelength of the incoming wave, the spreading-out will be smaller. If the gap is smaller than the wavelength, then the spreading-out will be very large. In a light microscope, the light waves will spread out whenever they pass through a lens, or any sort of obstruction. The primary lens in most microscopes is of much bigger than 300-900 nanometres, so the angle by which light waves diverge is really quite small. However, it is not zero the light waves do spread out a little bit, and the result is that the visual field is always a bit blurry. It is impossible for an ordinary light microscope to avoid this problem, so they can never see structures smaller than about 500 nm.
Yes, it is possible to shorten a wavelength by increasing the frequency of the wave. This relationship is described by the wave equation λ = c/f, where λ is wavelength, c is the speed of light, and f is frequency.
The resolving power of a microscope is inversely proportional to the wavelength of light being used. This means that as the wavelength of light decreases, the resolving power of the microscope increases. Shorter wavelengths can resolve smaller details, allowing for higher magnification and clearer images.
The transmission electron microscope operates on the same principle as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. Transmission electron microscopes use electrons as "light source" and their much lower wavelength makes it possible to get a resolution a thousand times better than with light microscope.
Changes in resolution with wavelength (light microscope) ... power improves as the wavelength of the illuminating light decreases. ...
The transmission electron microscope operates on the same principle as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. Transmission electron microscopes use electrons as "light source" and their much lower wavelength makes it possible to get a resolution a thousand times better than with light microscope.
Viruses. They are smaller than the average wavelength of light and as such are not viewable unless an electron microscope is used.
In a light microscope the resolution of the image it can project is limited by the distance each photon travels in its wavelength. Beneath this minimum distance, the "noise" of the photon's movement along its path overwhelms any resolution the light source may otherwise provide.
When you shorten the wavelength, the energy increases. This is because energy is inversely proportional to wavelength according to the equation E = hc/λ, where E is energy, h is Planck's constant, c is the speed of light, and λ is the wavelength. Shortening the wavelength increases the frequency, thus increasing the energy of the wave.
Viruses are too small to be seen with a light microscope because they are smaller than the wavelength of visible light. This means that light cannot pass through them or be reflected off of them, making them invisible under a light microscope.
Blue light provides the best resolution in a microscope because of its shorter wavelength compared to other colors in the visible light spectrum. The shorter wavelength allows for greater detail and resolution when viewing microscopic specimens.
== == When using a light microscope you encounter diffraction. (Visible light behaves like a wave, with a wavelength of about 300 to 900 nanometres). We say that light diffracts when its wavelike behaviour makes it bend around obstacles, or spread out. If the obstacle is much larger than the wavelength of the incoming wave, the spreading-out will be smaller. If the gap is smaller than the wavelength, then the spreading-out will be very large. In a light microscope, the light waves will spread out whenever they pass through a lens, or any sort of obstruction. The primary lens in most microscopes is of much bigger than 300-900 nanometres, so the angle by which light waves diverge is really quite small. However, it is not zero the light waves do spread out a little bit, and the result is that the visual field is always a bit blurry. It is impossible for an ordinary light microscope to avoid this problem, so they can never see structures smaller than about 500 nm.
No ten thousand is too far, given the wavelength of light, the limiting factor for optical microscopes. Perhaps a 1200 magnification is the practical limit for a simple light microscope.