Magnification refers to how much larger an object appears under the microscope compared to the naked eye, while field of view is the diameter of the area visible through the microscope lens at a given magnification. In simple terms, magnification is how big, and field of view is how much you can see.
As the magnification of a microscope increases, the diameter of the field of view decreases. This is because higher magnification allows for more detailed observation of objects, but with a narrower field of view. Conversely, lower magnification provides a wider field of view but with less magnification.
As magnification increases, the field of view decreases. This is because higher magnification enlarges the image being viewed, resulting in a smaller portion of the specimen being visible in the field of view. Conversely, decreasing magnification expands the field of view, allowing more of the specimen to be seen at once.
To switch between magnifications on a microscope and keep the same object in view, first center the object in your field of view at the lower magnification. Then, without moving the slide or stage, rotate the nosepiece to change to the desired higher magnification. The object should remain centered in your field of view at the new magnification. Adjust the focus as needed.
The effective focal length of a lens is the distance from the lens to the image sensor when the subject is in focus. The difference in effective focal length between two lenses affects the magnification and field of view of the images they produce. A longer focal length lens will have a narrower field of view and higher magnification, resulting in more detailed and compressed images. On the other hand, a shorter focal length lens will have a wider field of view and lower magnification, capturing more of the scene but with less detail. Ultimately, the choice of focal length depends on the desired composition and perspective of the image.
Short Answer: The unaided human eye which has a magnification of 1X and can resolve details down to about 0.2 millimeters. Longer Answer: Depending on the type of lens a single lens magnifier can magnify up to about 12X and resolve down to 17 micrometers. A multiple lens achromatic magnifier can go up to about 25X and resolves down to about 8 micrometers. Stereo microscopes that can easily view 3-D objects go up to about 1000X and resolve down to about 150 nanometers. Likewise, any optical microscope is limited by the diffraction limit of light to about 1300X of usable magnification. More magnification will just produce a larger version of the image that is blurry since it is not possible to sharpen the focus beyond the 150 nanometer diffraction limit. Microscopes that use ultraviolet light, which has a shorter wavelength that can focus tighter, can resolve down to 10s of nanometers or about 10 times better than visible light microscopes for a magnification of 10,000 X. Modern Scanning Electron Microscopes (SEM) are capable of resolving down to about 0.2 nanometers which translates to a useful magnification of 1,000,000 X. Specialized types of electron microscopes (Field Emission Transmission Electron Microscopes) can resolve down to 0.05 nanometers which translates to a magnification of 2,500,000 X. For reference, a carbon atom is about 15 nanometers in diameter.
As you increase the magnification, the field of view decreases.
As you increase the magnification, the field of view decreases.
Magnification is inversely proportional to the diameter of the field of view.
As the magnification of a microscope increases, the diameter of the field of view decreases. This is because higher magnification allows for more detailed observation of objects, but with a narrower field of view. Conversely, lower magnification provides a wider field of view but with less magnification.
both are bright field microscopes, and works on two lenses
Focal length refers to the distance between the lens and the image sensor, determining the field of view and magnification. Zoom, on the other hand, allows you to adjust the focal length within a range, changing the magnification and field of view without physically moving the camera.
As magnification increases, the field of view decreases. This is because higher magnification enlarges the image being viewed, resulting in a smaller portion of the specimen being visible in the field of view. Conversely, decreasing magnification expands the field of view, allowing more of the specimen to be seen at once.
Limitations of a dissecting microscope include limited magnification power (usually up to 50x), lower resolution compared to compound microscopes, and restricted depth of field which may limit the ability to view complex structures in detail. Additionally, the field of view can be smaller compared to other types of microscopes.
The higher the magnification the lower the depth of field.
Electron microscopes have a higher magnification and resolution compared to light microscopes, allowing for the visualization of smaller details in samples. They can also provide images with greater depth of field and contrast, enabling the study of subcellular structures in great detail.
Resolution: Electron microscopes have higher resolution than light microscopes. Magnification: Electron microscopes can achieve much higher magnifications than light microscopes. Wavelength of illumination: Electron microscopes use electrons for illumination, while light microscopes use visible light. Specimen preparation: Electron microscopes require more extensive specimen preparation compared to light microscopes. Depth of field: Light microscopes have a larger depth of field than electron microscopes. Cost and size: Electron microscopes are typically larger and more expensive than light microscopes.
Initially, the level of magnification possible with early microscopes, such as those developed in the late 16th and early 17th centuries, was around 20 to 30 times. Innovations by scientists like Antonie van Leeuwenhoek later increased magnification capabilities, allowing for up to 200 to 300 times. However, it wasn't until the advent of compound microscopes in the 19th century that magnifications exceeding 1000 times became achievable, significantly advancing the field of microscopy.