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As the name suggests there is an antigen - antibody reaction between the antigen (Hormone) of interest and its specific antibodies raised from laboratory animals. The quantum of antigen-antibody binding is monitored using a radiolabelled antigen or antibody. In brief the RIA kit contains test tubes which have been coated with the antibody. To these tubes, standards or samples are added (usually 25 to 200 ul volume) along with the tracer, The contents are incubated for 1 - 2 hours and the tubes are emptied by decanting the contents or aspirating. The tubes are washed with the wash solution provided and are counted in a gamma counter. The counts obtained for various known standards used in the assay are utilised for generating a dose - response curve and the unknown concentration in serum samples are extrapolated from the data. \
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Jeffrey C. Travis has written: 'Clinical radioimmunoassay' -- subject(s): Biochemistry, Clinical chemistry, Radioimmunoassay, Technique
How did Rosalyn Yalow impact society?
By invented the Radioimmunoassay
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Lubna Hameedabdullah has written: 'A radioimmunoassay for substance P'
What has the author Paul Franchimont written?
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What are the advantage of radioimmunoassay?
higher sensitivity, easy signal detection, and well-established, rapid assays
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the health and safety risks posed by the use of radiation and the time and expense associated with maintaining a licensed radiation safety and disposal program.
What has the author Janet Hedley written?
Janet Hedley has written: 'An evaluation of the comparable costs of commercial radioimmunoassay kits and alternative modes of reagent purchase'
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Sabitiu Alake Oyekele has written: 'Characterisation of a new antibody and the use of radioimmunoassay to study the effect of benserazide on bloodmelatonin'
Who is Rosalyn Sussman?
Rosalyn Sussman Yalow (1921-2011) was an American medical physicist, and a co-winner of the 1977 Nobel Prize in Medicine for her development of the radioimmunoassay process.
