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Why do specimens have to be in a vacuum in order to view them under a electron microscope?
Specimens have to be in a vacuum in order to view them under an electron microscope because electrons are easily scattered by air molecules. A vacuum environment provides a clear pathway for the electrons to travel without interference, allowing for high-resolution imaging of the specimen.
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How thin must a specimen be in order to view it on a microscope slide?
A typical thickness for a specimen on a microscope slide is about 0.1mm to 0.2mm. However, for some specialized applications, thinner sections may be required, such as in electron microscopy where specimens can be as thin as 50-100 nanometers. The key is to have a thin enough sample to allow light to pass through for accurate viewing.
Who invented the electron microscope?
German physist Ernst Bruche invented it with the help of his assistant, Ernst Ruska. Sadly, his assistant betrayed him, and went to work for someone else. Ernst Ruska got the Nobel Prize for his invention, but it should have belonged to Ernst Bruche.Some Electron Microscope History:It is widely accepted that the first PROTOTYPE of an electron microscope was built by Ernst Ruska and Max Knoll in 1931; it was not more powerful than an optical microscope, but it demonstrated the principle that is still used in today's sophisticated and powerful instruments, and earned its builders a share of a Nobel Prize. In 1933, Ruska built a a new prototype instrument that was more powerful than any optical microscope, but still just an experimental instrument.The first PRACTICAL electron microscope was not constructed until 1938, by Eli F Burton of the University of Toronto along with students Cecil Hall, James Hillier and Albert Prebus.There does not seem to be any literature linking Ernst Ruska to Ernst Bruche, nor any literature relating any claim by Bruche to developmental work on the electron microscope. Some bibliography is going to have to be provided in order for the sad tale of Ruska betraying Bruche to be credible.
What is a sentence for magnification?
We had to increase the magnification of the microscope in order to see the cells clearly.
Why a microscope is necessary in order to observe brownian motion?
A microscope is necessary to observe Brownian motion because the movement of particles is very small and needs to be magnified in order to be visible to the human eye. The microscope allows us to see the random motion of the particles as they collide with each other and move in a liquid or gas. Without a microscope, the individual particle movements would be too small to detect.
Why does specimen need to be thin?
Specimens need to be thin in order to allow light or electrons to pass through easily for proper visualization. Thinner specimens result in clearer images with better resolution, as the light or electrons can interact more effectively with the specimen.
Why might a microscopist who specializes in the use of microscopes to examine specimens use a light microscope instead of an electron microscope?
An electron microscope requires that the subject be dead to function. In order to receive a picture, the specimen must be coated in chemicals and put into a vacuum, a process which will kill any living specimen. A light microscope, although not as detailed, will allow the scientist to observe living specimens.
Can a scientist observe living things under an electron microscope?
No, electron microscopes require specimens to be in a vacuum chamber, which is not compatible with living organisms that need to be in a natural environment to survive. Instead, scientists typically use light microscopes to observe living organisms.
Why does the specimen on a microscope small?
Microscope specimens need to be small in order to allow light to pass through and be focused onto the eyepiece. Additionally, smaller specimens enable the microscope to magnify them effectively, making it easier to observe fine details. A smaller size also helps to ensure that the entire specimen remains in focus under the lens.
Why must a specimen be thin in order to be viewed under the microscope?
Specimens must be thin in order to be viewed under the microscope because light can only pass through a certain thickness of material. A thin specimen allows light to pass through and interact with the cells, allowing the microscope to produce a magnified image. Thicker specimens would prevent light from passing through and produce a blurry or unreadable image.
Which microscope should be ued for a virus is 50nm in size and why?
A virus of 50nm would be too small to see unless an electron microscope was used because it has greater resolving power and a resolution up to .1nm. A microscope using compound light as the means of illumination could not resolve better than approx. 200nms.
How thin must a specimen be in order to view it on a microscope slide?
A typical thickness for a specimen on a microscope slide is about 0.1mm to 0.2mm. However, for some specialized applications, thinner sections may be required, such as in electron microscopy where specimens can be as thin as 50-100 nanometers. The key is to have a thin enough sample to allow light to pass through for accurate viewing.
What is the purpose of a stage stop on a microscope?
A stage stop on a microscope can be used to limit the movement of the stage in order to control the area being viewed. It helps in keeping the sample in focus, especially when making precise adjustments or when observing multiple specimens.
Why does a doctor use a microscope?
A doctor uses a microscope to view cells, tissues, and other specimens at a magnified level in order to diagnose and treat diseases accurately. The microscope allows them to see details that are not visible to the naked eye, helping them make informed decisions about a patient's health.
Is flagella viewable through an electron microscop or a light microscope?
Flagella are typically only visible through an electron microscope due to their small size, which is on the order of a few micrometers. Light microscopes do not have the resolution necessary to clearly visualize structures at this scale.
What are applications of uncertainty principle?
Uncertainty Principle can be used to give a drawback to Bohr's Model of an atom. In that atomic model Bohr said that electrons exist in certain well defined energy levels, to give a contradiction to this statement uncertainty principle may be used.
Can mitochondria be seen under a light microscope or an electron microscope?
Mitochondria can be seen under an electron microscope due to their small size, which cannot be resolved by a light microscope. Electron microscopes have a higher resolution, allowing for the visualization of intracellular structures like mitochondria in greater detail.
What type of microscope will you use to examine the virus particle inside the brain cells?
Use of EM (electron microscope), because of the their high resolution power. In order to visualize Virus under EM they are either coated or stained with platinum or osmium (heavy metal) in such a way that the protein structure of the Virus is not destroyed. The particles are observed by passing electron through specimen and observing it in fluorescent screen. Area where electrons don't pass become black due to havy metal but are printed white because images are photographed in negative.
