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Why do you use a reagent blank and not just distilled water to zero the spectrophotometer?
Wiki User
∙ 12y ago
I know exactly what this question is from. This is an experiment testing the amount of dissolved iron in a solution, correct? If so, then this is the answer I came up with:
The solutions being tested are not simply iron dissolved in water; they contain other substances, like sulfuric acid, sodium citrate, etc. These other materials will affect the absorption of the sample solutions, so they must be zeroed out. Using only distilled water does not effectively account for this, so a reagent blank, having been treated in the same way as all of the other solutions, must be used instead.
This answer was not confirmed by my professor, but it is what I came up with, and it sounds reasonable to me. Hope that helps!
Mason Bobro
Wiki User
∙ 15y agoAS with any accurate machinery or scientific instrument there is a tendancy for them to get out of calibration over time, and after use. These products must meet specific requirements, codes, and standards. The purpose of "Zeroing" (or calibrating) is to make sure these items are brought back into "Zero" or "calibration" so that they can be as accurate as possible pertaining to the specifications that are required by the governing requirements, etc.. John Arndt/ CWI
Wiki User
∙ 12y agobecause a reagent blank is not as transparent as distilled water.if you use the distilled water to zero the spectrometer,the real absorbtion of reagent blank is not zero,so there should cause to error
Wiki User
∙ 11y agoyes
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What is the weight of distilled water?
Seawater weighs about 27 grams per litre more than fresh water.
Why is water a conductor?
Pure (distilled) water is not a good conductor; but most natural water has some ions, that help conductivity.
A ship is able to float on water because it has a blank density than the water?
Lower.
Is water a pure chemical substance?
Technically yes but there are usually impurities in it. Distilled water is very close to only containing H20.
Difference in conductivity between Distilled water and tap water?
Distilled water is water that has been vaporized and re-condensed and contains less (if not all) contaminants removed. Tap water has a whole host of chemicals in it due to additives, chemicals leeched into it from pipes, and chemicals left over from processing. But the primary difference is that tap water has natural minerals in it, such as calcium and magnesium, which can leave deposits in you teapot and on your shower head, but are actually healthier for drinking. Depending on what minerals and other chemicals are in the tap water, it can taste much better or much worse than distilled water, which has a flat taste.
If you want to read the absorbance at 595nm of a protein solution mixed with the colorizing solution called Bradford reagent what is a suitable reagent blank?
a "blank" cuvette can be made with a sufficient amount of distilled water (assuming you're diluting your stock solution with distilled water).
You calibrated the spectrophotometer with what?
Most likely a blank solution (water or buffer).
Is distilled water sufficient as a blank?
Don'y know
Why do we add water to reagent blank?
The reagent blank should contain everything that the sample contains, except one variable. That variable could be the active ingredient, the enzyme, the substrate, or some other ingredient that is essential to the reaction. If water is added to all the other tubes, it must also be added to the reagent blank.
What is the chemical content of Barritt's reagents A and B?
reagent A has ethanol and alpha-napthol reagent B has potassium hydroxide, creatine and distilled water
Why should you use ammonia-free distilled water on preparing of urea reagent?
i dont know soory
Why is it important to use seven up as your blank instead of distilled water?
It is not recommended.
What is blank in spectrophotometer?
A blank is used in order to cancel out or zero the absorbance of all the other components in the sample except the component whose absorbance is to be measured. For example, if you want to measure the absorbance of a solute in water, you will use only water as a blank and the spectrophotometer will subtract the absorbance of water from the spectrum when you measure the absorbance of your solute in water.
WHAT ISPasteur's salt solution?
Laboratory reagent consisting of potassium phosphate and calcium phosphate, magnesium sulfate, and ammonium tartrate in distilled water
What is the difference between reagent blank and sample blank?
Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
What is reagent water and distilled water?
Sterile or Sterilized Water - Water that meets the requirements under "Sterility Tests" in the United StatesPharmacopeia.Distilled Water - Water that is demineralized by distillation and complies with the requirements for purified water set forth in the United States PharmacopeiaDM water- Water that is demineralized by distillation, deionization, or reverse osmosis and complieswith the requirements for purified water set forth in the United States Pharmacopeia
Is it wfi used for inplace of lal reagent water?
The LAL reagent water can be sterile WFI or other water that show reaction with the specific LAL reagent with which it can be used, at the limit of the sensitivity of such reagent.
