The fine adjustment knob allows for more precise focusing adjustments under high power, where the depth of focus is much shallower. This helps in achieving sharp and clear images when observing fine details of the specimen. The coarse adjustment knob may be too sensitive and lead to overshooting the focus point under high magnification.
Using the course adjustment knob on high power can cause the microscope to move too quickly, potentially damaging the specimen or the objective lens. It is better to use the fine adjustment knob on high power for precise focusing.
The coarse adjustment knob moves the objective a lot while the fine adjustment knob moves it just a little, so using the coarse knob under high power can cause the objective to hit the slide and potentially damage the slide or the objective. The fine adjustment knob provides more precise control for focusing under high power, allowing for better clarity and accuracy.
The fine adjustment knob is used to bring the image of an object on the slide into focus when under the high power objective lens. This knob allows for precise focusing by making small adjustments to the distance between the objective lens and the specimen on the slide.
Course adjustment should not be used with high power because it can cause the microscope stage to move quickly and possibly crash into the objective lens or slide, causing damage. Additionally, using high power with course adjustment can result in a blurry image due to the rapid movement and lack of precision. It is better to use fine adjustment for precise focusing when using high power.
parfocal
When focusing the object under low power, you should use the coarse focus knob first to bring the object into general focus. Once the object is roughly in focus, then you can use the fine focus knob to sharpen the image.
course adjustment
The course adjustment moves the stage of the microscope further/closer away from the objective lens (it is used for general focusing), and the fine adjustment moves the objective lens very small distances for finer focusing.
You should find the 'adjustment knob'.
The adjustment knob that moves the body tube for focusing with the high-power objective lens is called the fine focus adjustment knob. It allows for precise adjustments to bring the specimen into sharp focus at high magnifications.
You should always use the fine adjustment on a microscope when you are focusing on high-power magnifications, typically 400x and above. The fine adjustment allows for precise focusing, which is crucial for observing details without risking damage to the slide or the lens. It is also recommended to use the fine adjustment after initially focusing with the coarse adjustment to enhance clarity.
You should find the 'adjustment knob'.
You should find the 'adjustment knob'.
You should find the 'adjustment knob'.
The working distance decreases when going from low power to high power magnification. This is because higher magnification lenses have shorter focal lengths, requiring the object to be placed closer to the lens to come into focus.
When focusing on a specimen under high power, you should use the fine adjustment knob. The coarse adjustment knob is typically used only under low power to prevent damage to the slide or objective lens. The fine adjustment allows for precise focusing, which is crucial at high magnifications to achieve a clear and sharp image of the specimen. Always ensure that you start with the lowest power objective before switching to high power for safety.
It is inappropriate to use the coarse adjustment knob when focusing on high-power objectives, typically 40x or higher, as this can lead to damage to the slide or the objective lens by bringing the stage too close to the lens. Instead, fine adjustment should be used for precise focusing at these magnifications. Coarse adjustment is suitable only for low-power objectives, where greater distances between the lens and slide are maintained.