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The speed at which pigment molecules move during paper chromatography is determined by their size, shape, and polarity. Smaller, less polar molecules will typically move faster than larger, more polar molecules because they can more easily pass through the pores of the paper. Additionally, the interactions between the pigment molecules and the solvent used in the chromatography process will also affect their speed of movement.

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Which determines the speed with which a pigment moves up the chromatography paper?

Chromatography is a technique that separates molecules from each other on the basis of their solubility in particular solvents. As a nonpolar solvent moves up the chromatography paper, the pigment moves along iwth it. The more non-polar a pigment, the more soluble it is in a nonpolar solvent, and the faster and father it proceeds up the chromatography. Pg 94, laboratory 8.1, Inquiry into Life, Sylvia S. Mader, laboratory manual, 12th edition


Which pigment move slowest in chromatogram?

In a chromatogram, the pigment that moves the slowest is typically chlorophyll b. This is because it has a higher affinity for the stationary phase (usually the paper or the gel) compared to other pigments, leading to less distance traveled. Other pigments, such as carotenoids, generally move faster due to their lower affinity for the stationary phase.


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