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Why taking culture OD at 660 NM?

Updated: 4/28/2022
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14y ago

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see od measures the light scattering and light scattering inversely varies with the wavelength. So smaller wavelength say 430nm would be more sensitive than longer wavelength say 660nm but here we take od in LB media and yellow colour absorbs shorter wavelngth so to obviate the error due to absorbance we take od at 660nm. -Satya Pal Arya

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14y ago
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Q: Why taking culture OD at 660 NM?
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