The magnification power of an optical microscope is limited by the wavelength of light used for imaging. Beyond a certain magnification level, the optical resolution becomes limited by the diffraction of light. This diffraction limit sets a maximum resolution that prevents higher magnifications from providing useful information.
one can make images of atoms using a scanning tunneling mcroscope.
Objective - Ocular 40 x 10 = 4000x
This depends on the type of microscope. Scanning tunneling microscopes have resolutions at the atomic level. Not that we can see the individual atoms and their structure in a scan. This is by todays technology impossible. (It is also thought to be impossible by future technology.) We can however quite accurately pinpoint the location of individual surface atoms in various metals and alloys. This helps us understand their structure combined. If we talk about the best of the best microscopes that uses visible light, then the best magnification we can hope to achieve is approx 1.500 times. The light and its wavelength is the problem when trying for more.
Yes, the wavelength of the light limits the maximum magnification of a microscope. Using visible light, the limit is about 1200 to 1500X.
one can make images of atoms using a scanning tunneling mcroscope.
A 1500X lens is used on a microscope to achieve high magnification, allowing you to see very small details of the specimen being viewed. This level of magnification is commonly used in scientific and medical research where precise examination of cells, tissues, or microorganisms is necessary. It helps in analyzing the fine structures and characteristics of the specimen with great clarity and detail.
Objective - Ocular 40 x 10 = 4000x
Several things do: 1) what magnification the ocular is (usually 10x) and the highest magnification of the objectives (usually 100x), giving you a total mag of 1000x 2) resolution, which in turn is affected by numerical aperture
The total magnification is equal to the magnification of the eyepiece multiplied by the magnification of the objective lens. So in this case the objective lens would need to be 100X.
This depends on the type of microscope. Scanning tunneling microscopes have resolutions at the atomic level. Not that we can see the individual atoms and their structure in a scan. This is by todays technology impossible. (It is also thought to be impossible by future technology.) We can however quite accurately pinpoint the location of individual surface atoms in various metals and alloys. This helps us understand their structure combined. If we talk about the best of the best microscopes that uses visible light, then the best magnification we can hope to achieve is approx 1.500 times. The light and its wavelength is the problem when trying for more.
18000
75 (1500x.05)
1500x 1.25
A light microscope uses a light source but a electron microscope has beams of electrons focused by magnetic lenses.- - -The major difference in practice is resolution: the amount of detail that can be discerned in the image.Under optimal conditions (excellent lenses, oil immersion) the resolution of a light microscope is about 0.2 micrometers (or 0.000,000,2 meters or 2 thousandths of a millimeter). Objects that are closer together cannot be detected as separate. The resolution of an transmission electron microscope is about 0.05 nanometers (or 0.000,000,000,05 meters or half of 1 millionth of a millimeter). This means that the electron microscope has about 10,000 times the resolving power of the light instrument, and can therefore show far greater detail, for example of subcellular structure.Another difference of importance to biologists is that light microscopes (there are several kinds, such as bright field and phase contrast) enable the viewer to watch living cells. The electron microscope produces an image of the shadows cast by atoms of heavy metals used as stains; the living tissue is destroyed by the intense beam of electrons.
http://www.fidelitysystems.com/Unlinked_DNA.html contains a photo of DNA obtained via an electronic microscope. Note added by another user: This is NOT a photo of DNA under a compound microscope. This is DNA under a scanning electron microscope.