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Judas had killed himself after his betrayal of Jesus. His replacement, Matthias (Acts 1:26), and all the other apostles were present.

Acts 2:1 - When the Day of Pentecost had fully come, they were all with one accord in one place. [NKJV]

(There's no "upper room" mentioned in connection with the Day of Pentecost. That was a different setting; where Jesus and the apostles kept the Passover, just before His betrayal. The Pentecost account just speaks of the "house where they were sitting.")

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