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The sex of tortoises is primarily determined by the temperature of the environment during the incubation of their eggs, a phenomenon known as temperature-dependent sex determination (TSD). Generally, higher temperatures tend to produce more females, while lower temperatures result in more males. This mechanism can vary among different species of tortoises, and specific temperature ranges can influence the ratio of males to females in a clutch. Other factors, such as genetics, may also play a role but are less significant than temperature.

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