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During his trip on the HMS Beagle, Charles Darwin observed variations in species among different environments, which led him to formulate his theory of evolution through natural selection. He also observed the diversity of flora and fauna in different regions, the impact of geographical isolation on species, and the adaptations of organisms to their environments.

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I just found this out from my professor and it is quite simply. The variations Darwin observed was the... Shapes, Lengths, and Color of the organisms. Hope this helps. -Jerry