What is perfusion culture?

Answer 1

Perfusion model is normally chosen for the production of unstable proteins. This is because perfusion culture has low resident time

in bioreactor and the product can be harvested and purified promptly. In addition, it can avoid the accumulation of toxic or

inhibitory metabolites that might have negative impact on the productivity of fed-batch cultures. Perfusion cultivation is carried out

by continuously feeding fresh medium to the bioreactor and constantly removing the cell-free spent medium while retaining the

cells in the reactor; thus, a much higher cell density can be obtained in perfusion cultures compared to continuous cultures, as cells

are retained within the reactor via a cell retention device. The perfusion rate depends on the demand of the cell line, the

concentration of nutrients in the feed, and the level of toxification.

Answer 2

Perfusion culture is a continuous cell culture method where fresh media is continuously supplied to and spent media is removed from the culture vessel. This allows for better control of nutrient supply, waste removal, and maintaining optimal conditions for cell growth and productivity. Perfusion culture is often used in bioprocessing for the production of biologics such as antibodies.