What is the meaning of rtkm?

Answer 1

RTKM stands for "Reads Per Kilobase of transcript, per Million mapped reads" and is a normalization method used in RNA sequencing data analysis. It quantifies gene expression levels by accounting for both the length of the gene and the total number of reads in the sample, allowing for comparisons between genes of different lengths and across different samples. This metric helps researchers understand the relative abundance of transcripts in various conditions or treatments.