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universal groups are not present in the win2000 mixed mode the forest level needs to be win2003 for it to work.

Universal groups can be used anywhere in the same Windows forest. They are only available in a Native-mode enterprise. Universal groups may be an easier approach for some administrators because there are no intrinsic limitations on their use. Users can be directly assigned to Universal groups, they can be nested, and they can be used directly with access-control lists to denote access permissions in any domain in the enterprise.

Universal groups are stored in the global catalog (GC); this means that all changes made to these groups engender replication to all global catalog servers in the entire enterprise. Changes to universal groups must therefore be made only after a careful examination of the benefits of universal groups as compared to the cost of the increased global catalog replication load. If an organization has but a single, well-connected LAN, no performance degradation should be experienced, while widely dispersed sites might experience a significant impact. Typically, organizations using WANs should use Universal groups only for relatively static groups in which memberships change rarely

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