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At Mission Santa Inés, the padres, or priests, were responsible for the spiritual and religious guidance of the Native American population, as well as the administration of the mission. They conducted daily Mass, provided religious education, and oversaw the conversion of Indigenous people to Christianity. Additionally, the padres managed agricultural activities and crafts, helping to establish the mission's self-sufficiency and fostering the development of the local economy. Their role was pivotal in the cultural exchange between the Spanish settlers and Native Americans.

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