How do you genetically engineer a bacteria?

Answer 1

To genetically engineer a bacterium, researchers typically use a vector, such as a plasmid, to introduce new DNA into the bacterial cells. This involves several steps: first, the desired gene is isolated and inserted into the plasmid, which is then introduced into the bacteria through methods like transformation, electroporation, or conjugation. Once inside, the bacteria can replicate the plasmid along with their own DNA, expressing the new gene and producing the desired protein or trait. Finally, the engineered bacteria are screened and selected for successful expression of the introduced genetic material.