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The missions in California had profound effects on the Indigenous populations. Firstly, they led to significant loss of land and resources, as vast areas were reallocated for mission use and agriculture, disrupting traditional lifestyles. Secondly, California Indians faced cultural assimilation and a decline in their languages and traditions due to missionary efforts to convert them to Christianity. Lastly, the introduction of European diseases decimated Indigenous populations, resulting in a drastic demographic shift and weakening of tribal societies.

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