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Mary, Queen of Scots, posed a significant threat to Elizabeth I due to her legitimate claim to the English throne as a Catholic monarch. Many Catholics viewed Elizabeth's rule as illegitimate, believing that Mary, being a direct descendant of Henry VII, had a stronger claim. Additionally, Mary's imprisonment and subsequent execution in 1587 heightened tensions, as it galvanized Catholic support against Elizabeth, leading to fears of rebellion and foreign intervention. The rivalry between their two factions intensified political instability in England.

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