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To make 1X PBS from 10X PBS, dilute the 10X PBS solution by mixing 1 part of 10X PBS with 9 parts of distilled water. For example, if you need 100 mL of 1X PBS, combine 10 mL of 10X PBS with 90 mL of distilled water. Mix thoroughly to ensure the solution is homogeneous. Store the diluted 1X PBS at room temperature or refrigerated for future use.

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AnswerBot

3d ago

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Related Questions

When to use 1x or 10x pbs?

Only use 1X PBS. 10X PBS is made up because it is easier to store and can be diluted when needed. Labs that use PBS often use it in large quantities so making up 10 or 20 liters at a time is usual and making it up as a concentrate is easier to do.


What does 1x PBS mean?

1X PBS might mean 1 in 10 solution 1/10


What is 10x - 1x?

10x - 1x = 9


10x PBS buffer?

10X PBS (0.1M PBS, pH 7.4): NaCl --------------------------------------------- 80 g KCl----------------------------------------------- 2g Na2HPO4 (anhydrous) ----------------------- 14.4 g KH2PO4 (anhydrous) ------------------------- 2.4g Distilled water ------------------------------- 1000 ml Mix to dissolve and adjust pH to 7.4 Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.


How do you convert 1x buffer to 10x buffer?

The stocks are commonly labeled as X factors such as 10X, 5X, 100X etc. X-factor indicates that the solution is concentrated and must be diluted usually with water to 1X concentration for use. For eg: - A 100X concentrated solution should be diluted to 100 fold. to convert 1X to 10X take one ml of 1x buffer in a measuring cylinder and dilute it to make it 10 ml. its now 10x buffer.


What is 1x PBS?

It is just Phosphate buffered saline.


What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?

1x PBS buffer typically has a molarity of around 0.01 M. To prepare a 20 mM PBS buffer, you would need to dilute the 1x PBS stock solution with water. For example, to make 1 liter of 20 mM PBS buffer, you would need to mix 2 ml of 1 M PBS stock solution with 98 ml of water.


What is the answer to 9x plus x?

9x + 1x = 10x.


.1x equals 2 what is x?

incomplete formula.1x of 20 equals 2 = .10x is 0.1x?=2 ?=20


What is X if X plus .1X equals 110?

x+0.1x=110 10x+1x=1100 11x=1100 x=100


Help you with algebra -10x equals 80?

10x=80 divide each side by 10 to get x by itself 10/10x=1x 80/10=8 x=8


What is -11x-1 equals -10x-8?

-11x-1 = -10x-8 -11x+10 = -8+1 -1x = -7 x = 7